Abstract：Myostatin (Mstn) gene is a well-studied and well-prospected muscle development inhibitory factor.Mstn gene has a wide range of endocrine characteristics, which not only regulate muscle development, but also regulate the metabolic processes such as glucose, lipids and amino acids. In this study, the Mstn gene-edited cattle (Bos taurus) and normal control were used to detect the changes of key metabolites in serum under the influence of exercise and feeding. The results showed that the fasting blood glucose of Mstn-/+ cattle was significantly lower than that of the control group (P<0.05), After exercise, the blood glucose levels of Mstn-/+ cattle and control cattle decreased significantly, and the Mstn gene mutation accelerated this changed. The serum cholesterol, triglyceride, high density lipoprotein, low density lipoprotein content changes relatively stable, neither exercise nor feeding could change the difference trend of Mstn-/+ cattle and control cattle. The test results of free fatty acids in serum showed that under fasting state, exercise would significantly reduced the content of saturated fatty acids in Mstn-/+ bovine serum and increased the level of unsaturated fatty acids (including monounsaturated fatty acids and polyunsaturated fatty acids). From the perspective of amino acid levels in serum, the Mstn mutation increased the concentration of essential and non-essential amino acids in bovine plasma, but exercise and food intake would not significantly affect the level of amino acids in serum. The results of this study have certain guiding significance for in-depth understanding of the function of Mstn gene and exploring the metabolic regulation mechanism of Mstn.
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