Cloning, Tissue Expression of PTEN Gene in Muscovy Duck (Cairna moschata) and Its Localization in Follicles
LIN Jun-Yuan1, NIU Yu-Rui1, MEI Xiang1, CHEN Chu1, GUO Shun2, HU Zhi-Gang1, LIU Xiao-Lin1*
1 College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China; 2 Animal Husbandry Development Center of Tongguan County, Tongguan 714300, China
Abstract：The phosphatase and tensin homologue deleted on chromosome 10 (PTEN) is a tumor suppressor gene. It plays an important role in cell growth, organ development and metabolism. In this study, the PTEN gene (GenBank No. OQ359981) was cloned using cDNA of Muscovy duck (Cairna moschata) ovary tissue as template, and bioinformatics analysis was performed. It was found that the full length of PTEN gene CDS was 1 212 bp, and the coded amino acid sequence was highly conserved. The expression of PTEN gene and protein in the kidney, liver, spleen, lung, heart, ovary and follicles of different grades were detected by qRT-PCR and Western blot. The results showed that PTEN was expressed in all the tissues, especially in ovary, spleen and heart; PTEN was expressed in both pre-hierarchical follicles and pre-ovulatory follicles, with the highest expression in small yellow follicles (SYF); Immunohistochemical staining confirmed that PTEN located in the granulosa cell layer of follicles. The above results suggest that the PTEN gene might be involved in the growth and development of duck follicles. The present study provides basic materials for further exploration of the regulation mechanism of PTEN gene in follicle development, and also provides new ideas for the study of duck genetic breeding.
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