Expression Analysis of StTCP23 Gene in Potato (Solanum tuberosum) Tubers During Dormancy Release
WANG Kai-Tong1, PU Xue1, WANG Rui1, ZHANG Huan-Huan2,3, LIU Sheng-Yan3, WANG Xiao1, ZHANG Ning1,2,*, SI Huai-Jun1,2
1 College of Life Science and Technology, Gansu Agricultural University, Lanzhou 730070, China; 2 State Key Laboratory of Aridland Crop Science, Lanzhou 730070, China; 3 College of Agronomy, Gansu Agricultural University, Lanzhou 730070, China
Abstract：TCP (teosintebranchⅠ, cycloidea, proliferating cell factors 1 and 2) is a class of plant-specific transcription factors and play an important regulatory role in plant growth and development. Dormancy and sprouting of potato (Solanum tuberosum) tuber is extremely important for potato breeding, tuber production and processing. Our previous study found that the expression of the StTCP23 (GenBank No. NW _ 006239107.1) in potato was continuously up-regulated in the process of tuber dormancy release. To clarify the biological function of the StTCP23, this study carried out cloning, subcellular localization, and bioinformatics analysis, and used qPCR technology to analyze its tissue specificity and expression during tuber dormancy release. The results showed that StTCP23 was mainly located in the nucleus. StTCP23 contained a typical TCP conserved domain, belonging to non-transmembrane proteins, and the total phosphorylation site accounted for 22.44%. The expression of StTCP23 gene was the highest in potato leaves and there was a variety difference. The expression level of StTCP23 gene showed a continuous upward trend from dormancy to sprouting of tubers, and the expression difference of StTCP23 gene was extremely significant at dormancy and dormancy release stages (P<0.01). The results provide a basis information for elucidating the function of StTCP23 gene in potato.
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