Abstract：Aldehyde dehydrogenases (ALDHs) play an important role in aldehyde stabilization under abiotic stress in plants.The members of ALDH superfamily in tea plant (Camellia sinensis) were identified by bioinformatics methods, and the biological information such as physicochemical properties, chromosome localization, gene structure, promoter cis-acting elements and phylogenetic relationship of the encoded proteins were analyzed. The expression patterns of the CsALDH superfamily members under drought, salt, and cold stresses ware analyzed by qRT-PCR. The results showed that a total of 27 ALDH superfamily members were identified in the C. sinensis genome, and phylogenetic analyses classified them into 10 families. Family members exhibited comparable motif composition and gene structure. The co-linearity analysis indicated that CsALDHs genes underwent gene duplication events in the C. sinensis genome, which experienced purifying selective pressures.The qRT-PCR results showed that 8, 10 and 9 candidate CsALDHs genes were induced by PEG, NaCl and low temperature, respectively. In particular, CsALDH2B4 and CsALDH3H2 were significantly up-regulated. This study provides a theoretical foundation for further research on the functions of ALDH genes in tea plants.
[1] Black W, Vasiliou V.2009. The aldehyde dehydrogenase gene superfamily resource center[J] Human Genomics, 4: 136. [2] Cao Q H, Lv W Y, Jiang H, et al.2022. Genome-wide identification of glutathione S-transferase gene family members in tea plant (Camellia sinensis) and their response to environmental stress[J]. International Journal of Biological Macromolecules, 205: 749-760. [3] Cao Y X, Wang J, Zhao S Q, et al.2023. Overexpression of the aldehyde dehydrogenase AhALDH3H1 from Arachishypogaea in soybean increases saline-alkali stress tolerance[J]. Frontiers in Plant Science, 14: 1165384. [4] Chen C J, Wu Y, Li J W, et al.2023a. TBtools-II: A 'one for all, all for one' bioinformatics platform for biological big-data mining[J]. Molecular Plant, 16(11): 1733-1742. [5] Chen J K, Gang G, Yu C M, et al.2020. Ramie BnALDH genes and their potential role involved in adaptation to hydroponic culturing condition[J]. Industrial Crops and Products, 157: 112928. [6] Chen Y Y, Wu X, Wang X H, et al.2023b. PusALDH1 gene confers high levels of volatile aroma accumulation in both pear and tomato fruits[J]. Journal of Plant Physiology, 290: 154101. [7] Du H M, Lee C, Jin X W, et al.2022. Overexpression of the aldehyde dehydrogenase gene ZmALDH confers aluminum tolerance in Arabidopsis thaliana[J]. International Journal of Molecular Sciences, 23(1): 477. [8] Gao C X, Han B.2009. Evolutionary and expression study of the aldehyde dehydrogenase (ALDH) gene superfamily in rice (Oryza sativa)[J]. Gene, 431(1): 86-94. [9] Gautam R, Ahmed I, Shukla P, et al.2019. Genome-wide characterization of ALDH superfamily in Brassica rapa and enhancement of stress tolerance in heterologous hosts by BrALDH7B2 expression[J]. Scientific Reports, 9(1): 7012-7012. [10] Goodstein D M, Shu S, Howson R, et al.2012. Phytozome: A comparative platform for green plant genomics[J]. Nucleic Acids Research, 40(Database issue): D1178-1186. [11] Hou Q C, Bartels D.2015. Comparative study of the aldehyde dehydrogenase (ALDH) gene superfamily in the glycophyte Arabidopsis thaliana and Eutrema halophytes[J]. Annals of Botany, 115(3): 465-479. [12] Islam M S, Ghosh A.2022. Evolution, family expansion, and functional diversification of plant aldehyde dehydrogenases[J]. Gene, 829: 146522. [13] Islam M S, Hasan M S, Hasan M N, et al.2021. Genome-wide identification, evolution, and transcript profiling of aldehyde dehydrogenase superfamily in potato during development stages and stress conditions[J]. Scientific Reports, 11(1): 18284. [14] Islam M S, Mohtasim M, Islam T, et al.2022. Aldehyde dehydrogenase superfamily in sorghum: Genome-wide identification, evolution, and transcript profiling during development stages and stress conditions[J]. BMC Plant Biology, 22(1): 316. [15] Jimenez-Lopez J C, Gachomo E W, Seufferheld M J, et al.2010. The maize ALDH protein superfamily: Linking structural features to functional specificities[J]. BMC Structural Biology, 10(1): 43-43. [16] Jimenez-Lopez J C, Lopez-Valverde F J, Robles-Bolivar P, et al.2016. Genome-wide identification and functional classification of tomato (Solanum lycopersicum) aldehyde dehydrogenase (ALDH) gene superfamily[J]. PLOS ONE, 11(10): e0164798. [17] Ke Y G, Yuan M, Liu H B, et al.2020. The versatile functions of OsALDH2B1 provide a genic basis for growth-defense trade-offs in rice[J]. Proceedings of the National Academy of Sciences of the USA, 117(7): 3867-3873. [18] Kim J, Shiu S H, Thoma S, et al.2006. Patterns of expansion and expression divergence in the plant polygalacturonase gene family[J]. Genome Biology, 7(9): R87. [19] Kirch H H, Bartels D, Wei Y L, et al.2004. The ALDH gene superfamily of Arabidopsis[J]. Trends in Plant Science, 9(8): 371-377. [20] Li L L, Yang L, Fan D Y, et al.2023. Genome-wide analysis of ALDH gene family in jujube and identification of ZjALDH3F3 for its important role in high-temperature tolerance[J]. Plant Physiology and Biochemistry, 205: 108196. [21] Li P H, Xia E H, Fu J M, et al.2022. Diverse roles of MYB transcription factors in regulating secondary metabolite biosynthesis, shoot development, and stress responses in tea plants (Camellia sinensis)[J]. The Plant Journal, 110(4): 1144-1165. [22] Li Y Y, Wang X W, Ban Q Y, et al.2019. Comparative transcriptomic analysis reveals gene expression associated with cold adaptation in the tea plant Camellia sinensis[J]. BMC Genomics, 20(1): 624. [23] Liang Y, Yu Y J, Cui J L, et al.2016. A comparative analysis of the evolution, expression, and cis-regulatory element of polygalacturonase genes in grasses and dicots[J]. Functional & Integrative Genomics, 16(6): 641-656. [24] Munim T B, Jameel I L, Al-Shammari R H H, et al.2023. Identification and characterization of aldehyde dehydrogenase (ALDH) gene superfamily in garlic and expression profiling in response to drought, salinity, and ABA[J]. Gene, 860: 147215. [25] Shen C, Li X.2023. Genome-wide identification and expression pattern profiling of the ATP-binding cassette gene family in tea plant (Camellia sinensis)[J]. Plant Physiology and Biochemistry, 202: 107930. [26] Shin J H, Kim S R, An G.2008. Rice aldehyde dehydrogenase7 is needed for seed maturation and viability[J]. Plant Physiology, 149(2): 905-915. [27] Su W H, Zhang C, Wang D J, et al.2021. A comprehensive survey of the aldehyde dehydrogenase gene superfamily in Saccharum and the role of ScALDH2B-1 in the stress response[J]. Environmental and Experimental Botany, 194: 104725. [28] Tian F X, Zang J L, Wang T, et al.2015. Aldehyde dehydrogenase gene superfamily in Populus: Organization and expression divergence between paralogous gene pairs[J]. PLOS ONE, 10(4): e0124669. [29] Tola A J, Jaballi A, Germain H, et al.2020. Recent development on plant aldehyde dehydrogenase enzymes and their functions in plant development and stress signaling[J]. Genes, 12(1): 51. [30] Wei C L, Yang H, Wang S B, et al.2018. Draft genome sequence of Camellia sinensis var. sinensis provides insights into the evolution of the tea genome and tea quality[J]. Proceedings of the National Academy of Sciences of the USA, 115(18): E4151-E4158. [31] Wu Q, Tong W, Zhao H J, et al.2022. Comparative transcriptomic analysis unveils the deep phylogeny and secondary metabolite evolution of 116 Camellia plants[J].The Plant Journal: For Cell and Molecular Biology, 111(2): 406-421. [32] Xu J, Liu L X, Huang H, et al.2023. Genome-wide characterization and gene expression analyses of ALDH gene family in response to drought stress in moso bamboo (Phyllostachys edulis)[J]. Plant Physiology and Biochemistry, 202: 107954. [33] Yang H L, Zhang D W, Zhang D Y, et al.2019. Overexpression of ALDH21 from Syntrichia caninervis moss in upland cotton enhances fiber quality, boll component traits, and physiological parameters during deficit irrigation[J]. Crop Science, 59(2): 553-564. [34] Yang H Y, Zhang D Y, Li X S, et al.2016. Overexpression of ScALDH21 gene in cotton improves drought tolerance and growth in greenhouse and field conditions[J]. Molecular Breeding, 36(3): 1-13. [35] Yang M Y, Teng Y H, Yue T, et al.2023. The Overexpression of peanut (Arachis hypogaea L.) AhALDH2B6 in soybean enhances cold resistance[J]. Plants, 12(16): 2928. [36] Zhang Q, Cai M C, Yu X M, et al.2017. Transcriptome dynamics of Camellia sinensis in response to continuous salinity and drought stress[J]. Tree Genetics & Genomes, 13(4): 78. [37] Zhang W Y, Zhang Y J, Qiu H J, et al.2020. Genome assembly of wild tea tree DASZ reveals pedigree and selection history of tea varieties[J]. Nature Communications, 11(1): 3719. [38] Zhao Z F, Song Q F, Bai D C, et al.2022. Population structure analysis to explore genetic diversity and geographical distribution characteristics of cultivated-type tea plant in Guizhou Plateau[J]. BMC Plant Biology, 22(1): 55.
