Identification and Expression Analysis of HMGR Gene Family in Jujube (Ziziphus jujuba)
LI Na1, HAN Hong-Yan1, SONG Yu-Qin2, FENG Xin-Xin2, LI Liu-Lin2*, LI Jie3, HUA Yan-Bin4
1 Department of Biological Sciences and Technology, Jinzhong University, Jinzhong 030619, China; 2 College of Horticulture, Shanxi Agricultural University, Jinzhong 030801, China; 3 College of Forestry, Shanxi Agricultural University, Jinzhong 030801, China; 4 Jiexiu Agriculture and Rural Bureau, Jinzhong 032000, China
Abstract：Terpenoids are the main substances for the medicinal function of jujube (Ziziphus jujuba). 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR) plays an important role in the biosynthesis of plant terpenoids. In order to further study the function of jujube HMGR genes, this study used HMM to identify the jujube HMGR gene family. Bioinformatics analysis software was used to analyze the physicochemical characteristics, gene structure, and evolutionary relationship of ZjHMGR genes. qRT-PCR was used to analyze the expression pattern of HMGR genes. The results showed that there were 21 HMGR gene members in the jujube genome, which were named ZjHMGR1~ZjHMGR21 according to their positions on the chromosome. The 21 HMGR genes were located on 10 chromosomes and 2 unplaced genomic scaffolds. The open reading frame (ORF) length of the ZjHMGR genes was 271 to 3 425 bp, encoding 88 to 1 122 amino acids, and the molecular weight ranged from 9 827.05 to 124 950.93 kD. The phylogenetic analysis of jujube and arabidopsis (Arabidopsis thaliana), apple (Malus pumila), pear (Pyrus bretschneideri), tobacco (Nicotiana tabacum) and watermelon (Citrullus lanatus) showed that the ZjHMGR1 was a separate group, while the ZjHMGR11 and ZjHMGR12, ZjHMGR5 and ZjHMGR10 were classified as separate class. And these 5 gene family members had collinear relationships with arabidopsis, apple, pear and watermelon. There were hormone-related cis-elements in the 2.0 kb promoter region of ZjHMGR gene, among which 11 candidate genes had gibberellin, methyl jasmonate and abscisic acid response cis-elements, 8 candidate genes had auxin response cis-elements, and 12 candidate genes have salicylic acid response cis-elements. Compared with young fruits, the expression level of ZjHMGR gene in mature fruits was significantly increased. The expression levels of the ZjHMGR genes in 'Linhuang No.1' and 'Muzao' fruits were significantly negatively correlated with the contents of chlorophyll a, chlorophyll b, total chlorophyll and anthocyanins, but positively correlated with the contents of carotenoids.ZjHMGR played an important role in fruit ripening. This study provides reference for genetic improvement of the synthesis of jujube terpenoids.
李娜, 韩红艳, 宋宇琴, 冯新新, 李六林, 李洁, 化延斌. 枣HMGR基因家族鉴定及表达分析[J]. 农业生物技术学报, 2025, 33(4): 743-754.
LI Na, HAN Hong-Yan, SONG Yu-Qin, FENG Xin-Xin, LI Liu-Lin, LI Jie, HUA Yan-Bin. Identification and Expression Analysis of HMGR Gene Family in Jujube (Ziziphus jujuba). 农业生物技术学报, 2025, 33(4): 743-754.
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