Cloning and Expression Analysis of DGK Gene Family in Potato (Solanum tuberosum)
WANG Di, HE Shuang-Shuang, SHAN Ya-Cheng, HUANG Yan, ZENG Xiang-Qin, TIAN Zhou-E, HU Xin-Xi, QIN Yu-Zhi, LIN Yuan*
College of Horticulture, Hunan Agricultural University/Hunan Provincial Potato Engineering and Technology Research Centre/Key Laboratory for Vegetable Biology of Hunan Province, Changsha 410128, China
Abstract：Diacylglycerol kinase (DGK) is a key enzyme in lipid signalling pathways and plays an important role in phytohormone signalling, biotic and abiotic stresses. This study analysed the sequence characteristics of the StDGKs genes in potato (Solanum tuberosum) with reference to the protein sequence of the model plant Arabidopsis thaliana AtDGKs as well as information on the conserved structural domains of DGK, and sequence cloning and analysis of this multigene family were performed, followed by analysis of its expression pattern in response to drought, salt stress and potato tuber development. The results showed that 11 StDGKs were screened from the potato genome database (S. tuberosum group Phureja DM1-3 v6.1) and 6 of them (StDGK1, StDGK2, StDGK5, StDGK6, StDGK7, StDGK8) were successfully cloned. Sequence analysis showed that the full length of the 6 StDGKs were 1 460~2 220 bp, and the similarity of sequence comparison was more than 95%. Real-time fluorescence quantification PCR showed that the expression of 6 StDGKs were induced by drought and salt stress. Compared with the control, the expression of StDGK2 and StDGK8 were significantly up-regulated and peaked at 12 h of drought treatment and StDGK6 at 4 h. The expression of StDGK1, StDGK5, StDGK7 were significantly higher than that of the control under salt stress treatment and peaked at 4 h, and that of StDGK2 and StDGK6 peaked at 15 min of salt treatment. In addition, this study found that StDGKs played an important role in the early stage of potato tuber development. For example, the expression of StDGK1 and StDGK8 gradually increased from the early to the middle stage of tuber development, and then slowly decreased at the late stage of tuber expansion. This study provides basic materials for a deeper understanding of StDGKs gene function.
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