摘要NAC (NAM, ATAF1/2, CUC2)转录因子是植物特有的一类转录因子超家族,在植物发育生长和逆境胁迫响应方面发挥重要作用。本研究从甘蓝型油菜(Brassica napus)中克隆得到BnaNAC069基因,序列分析和系统发育分析显示,BnaNAC069的氨基端有1个经典的NAC结构域,羧基端包含1个跨膜结构域,是1个典型的膜结合NAC转录因子,与拟南芥(Arabidopsis thaliana)中的AtNTL13/ANAC069亲缘关系最近。亚细胞定位分析显示,BnaNAC069定位于内质网膜上;表达模式分析表明,高盐(NaCl)、水杨酸(salicylic acid, SA)、过氧化氢(H2O2)处理可以显著诱导BnaNAC069的表达。表型分析发现,anac069拟南芥突变体植株对高盐胁迫耐受,过表达BnaNAC069的甘蓝型油菜对干旱和高盐敏感。qRT-PCR结果表明,BnaNAC069抑制脱水诱导(responsive to dehydration)基因BnaRD29B和BnaAREB1 (ABA responsive element binding protein 1)的表达水平,表明BnaNAC069可能通过ABA依赖的途径负调控植物对干旱和盐胁迫的响应。本研究为深入探讨甘蓝型油菜中BnaNAC069基因在调控对干旱和高盐逆境过程中的作用机制提供了基础资料。
Abstract：NAC (NAM, ATAF1/2, CUC2) transcription factors are a superfamily of transcription factors that are unique to plants and play an important role in plant development and stress response. In this study, BnaNAC069 was cloned from Brassica napus. Sequence analysis and phylogenetic analysis revealed that BnaNAC069 had a classical NAC domain in its N-terminal and a transmembrane domain in its C-terminal, which was a typical membrane-bound NAC transcription factor. BnaNAC069 had the closest genetic relationship with Arabidopsis thaliana AtNTL13/ANAC069. Subcellular localization showed that BnaNAC069 was located on the endoplasmic reticulum membrane. The expression pattern analysis showed that high salinity (NaCl), salicylic acid (SA) and hydrogen peroxide (H2O2) could significantly induce the expression of BnaNAC069. Phenotypic analysis showed that the A. thaliana anac069 mutants were tolerant to high salinity, while plants overexpressing BnaNAC069 were sensitive to drought and high salinity. The results of qRT-PCR showed that BnaNAC069 negatively regulated the expression of BnaRD29B (responsive to dehydration) and BnaAREB1 (ABA responsive element binding protein 1), suggesting that BnaNAC069 negatively modulates plant response to drought and high salinity in an ABA-dependent pathway. This study provides basic data for further investigation of the function of the BnaNAC069 gene in regulating plant response to drought and salt stress in B. napus.
舒琳, 李龙辉, 曹亚男, 王敖民, 于忠晨, 燕敬利. 甘蓝型油菜NAC069基因的克隆与功能分析[J]. 农业生物技术学报, 2024, 32(12): 2701-2714.
SHU Lin, LI Long-Hui, CAO Ya-Nan, WANG Ao-Min, YU Zhong-Chen, YAN Jing-Li. Cloning and Functional Analysis of NAC069 Gene in Canola (Brassica napus). 农业生物技术学报, 2024, 32(12): 2701-2714.
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