Abstract：Strawberry gray mould, caused by Botrytis cinerea, is a highly prevalent and severely damaging disease in strawberry(Fragaria × ananassa) cultivation. Trichoderma spp. is a currently widely utilized and highly effective biological control agent. To effectively control strawberry gray mould using Trichoderma, in this study, the plate dilution method and confrontation test were used to screen Trichoderma strains that inhibit B. cinerea. The strains were identified through morphological characteristics and molecular biological analysis. The biocontrol mechanism, pot control effects of Trichoderma czit against strawberry gray mould and growth-promoting effects on strawberry seedlings were investigated by plate culture, microscopy observation and root drenching methods. The results showed that Trichoderma czit was obtained from healthy strawberry rhizosphere soil, which had a strong inhibitory effect on B. cinerea, with an inhibition rate of 80.35%. By combining morphological features and molecular biology methods, strain czit was identified as T. longibrachiatum. The results of the biocontrol mechanism indicated that, T. longibrachiatum czit had obvious competitive, antibiotic and hyperparasitism effects on B. cinerea. czit could rapidly occupy nutrient space, completely cover B. cinerea after 7 d of confrontation culture. The inhibitory rate of its volatile metabolites against B. cinerea was 64.12%, and that of its fermentation metabolites was 72.00%. The mycelium of czit could play the role of hyperparasitism by attaching, entangling, penetrating, and deforming B. cinerea mycelium. The results of the pot experiment showed that the strain czit had a 66.26% pot control effect on strawberry gray mould, and had a significant growth-promoting effect on strawberry seedlings. The promoting rates of plant height, root length, total fresh weight, root fresh weight, shoot fresh weight, and root dry weight of strawberry seedlings were 35.82%, 36.80%, 105.34%, 96.63%, 116.22%, and 102.08%, respectively. T. longibrachiatum czit selected in this study had significant control effects on strawberry gray mould and had a significant growth-promoting effect on strawberry seedlings, demonstrating good biocontrol potential and application prospects.
张克胜, 刘萍, 卢明媚, 金辉, 霍洋棋, 李想, 杨瑞先. 长枝木霉czit防控草莓灰霉病的作用机制及植株促生功能研究[J]. 农业生物技术学报, 2026, 34(8): 1726-1737.
ZHANG Ke-Sheng, LIU Ping, LU Ming-Mei, JIN Hui, HUO Yang-Qi, LI Xiang, YANG Rui-Xian. Study on the Mechanism of Trichoderma longibrachiatum czit Against Strawberry Gray Mould and Its Growth-promoting Function on Plants. 农业生物技术学报, 2026, 34(8): 1726-1737.
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