Abstract：Phosphorus in soil exists in complex forms, such as phytic acid, which is difficult to be absorbed and utilized by plants. This problem can be effectively solved through degrading soil phytic acid by phytase and releasing phosphorus. In this study, high-yielding phytase strains were selected from the soil, and the composition of the fermentation medium and culture conditions were optimized by the Response Surface Methodology. Phytase was prepared into microcapsule enzyme preparation with sodium alginate (SA) and CaCl2 as embedding and crosslinking agents, and its storage stability and growth-promoting effect on corn (Zea mays) were investigated. The results showed that a strain of phytase-producing bacteria was identified as Pseudomonas corrugata by 16S rRNA. The optimal medium composition and fermentation conditions were determined through fermentation optimization, and the phytase activity after optimization was (435.70±1.87) U/mL, which was increased by 82.16% compared with that before optimization. The microcapsule enzyme preparation prepared with 3% SA and 3% CaCl2 had the characteristics of good pellet formation and high enzyme activity. After 30 d of storage, the enzyme activity of (85.63±1.73)% remained. The experiment showed that microcapsule enzyme preparation significantly promoted corn growth, increasing corn seedling dry weight, plant height, root dry weight, root length and fresh seedling weight by 48.59%~304.46%. This study provided a method of strain screening, fermentation optimization and preparation of microcapsule enzyme preparation, which is of great significance for the extensive application of microcapsule enzyme preparations in agricultural production and new bacterial fertilizers.
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