1 College of Marine and Biotechnology/Guangxi Key Laboratory for Polysaccharide Materials and Modifications, Guangxi University for Nationalities, Nanning 530008, China; 2 Guangxi Lvyounong Biological Technology Co., Ltd., Nanning 537100, China
Abstract：The banana (Musa acuminata) anthracnose disease caused by Colletotrichum musae is one of the most common post harvest diseases of bananas. To screen biocontrol bacterial strains with significant efficacy against banana anthracnose, in this study, a strain, Y-2-3, was isolated from soil samples collected from an abandoned salt field in Beihai, Guangxi, China, showing an inhibition rate of 75.62% against C. musae, which mycelia treated with Y-2-3 became swollen, deformed, and intertwined. Functional characterization revealed that Y-2-3 possesses growth-promoting properties, including nitrogen fixation, ammonia (NH3) production, and phosphate solubilization, as well as the ability to produce cellulase and amylase. 16S rRNA and gyrase subunit B (gyrB) gene sequencing identified Y-2-3 as Bacillus velezensis. Dual culture assays confirmed that Y-2-3 exhibited broad-spectrum antifungal activity, with inhibition rates exceeding 50% against multiple pathogenic fungi. Moreover, volatile compounds produced by Y-2-3 exhibited a strong inhibitory effect on C. musae. Postharvest preservation experiments demonstrated that banana disease lesions were controlled within 40% under volatile treatment with Y-2-3. In conclusion, strain Y-2-3 exhibits both growth-promoting and biocontrol properties, showing great potential for application in banana anthracnose management and sustainable agriculture. This study provides theoretical basis and high-quality bacterial resources for the biological control of banana anthracnose.
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