Control of Wheat Sharp Eyespot by Combination of Bacillus subtilis Z-14 Spore Preparation and Kuras
ZHANG Jing-Jing, WANG Min, ZHAO Gang-Yi, BIAN Yin-Bo, LIU Na, ZHANG Dong-Dong, HOU Chun-Yan, HAN Sheng-Fang*, WANG Dong-Mei*
State Key Laboratory of North China Crop Improvement and Regulation/Key Laboratory of Hebei Province for Plant Physiology and Molecular Pathology/College of Life Sciences, Hebei Agricultural University, Baoding 071001, China
Abstract：In recent years, the occurrence of wheat sharp eyespot has been increasing year by year, seriously affects food security. The prevention and control of wheat (Triticum aestivum) diseases in production mainly relies on chemical pesticides, but chemical control has many problems. In this paper, plate antagonism test, virulence assay and compatibility assay were used to preliminarily analyze the inhibition effect of fermentation broth of Bacillus subtilis Z-14 strain on the mycelial growth of Rhizoctonia cerealis, the virulence of pesticide Kuras on Rhizoctonia cerealis and the compatibility between Kuras and Z-14 strain. On this basis, the spore preparation of strain Z-14 (8.5×107 CFU/mL) combined with pesticide Kuras seed dressing on wheat sharp blight control was studied. The results showed that Kuras had high virulence against Rhizoctonia cerealis, and its average concentration for 50% of maximal effect (EC50) value of inhibiting the mycelial growth of Rhizoctonia cerealis was 0.035 μg/mL; At this concentration, there was no obvious inhibition on the growth of Z-14 bacteria, and it had good compatibility; The inhibition rate of Z-14 fermentation liquid on mycelia growth of wheat sharp eyespot was 86.57%. In greenhouse, the combined seed dressing of Z-14 spore preparation and Kuras had a control effect of 46.12% against sharp blight at the jointing stage of wheat, which was significantly higher than that of the single component. The combined use also promoted growth indicators such as root length, plant height, and stem thickness. Therefore, the use of biological agents to control wheat diseases is effective, it is of great significance for promoting the green development of agriculture.
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