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2025年8月13日 星期三
农业生物技术学报  2024, Vol. 32 Issue (4): 859-872    DOI: 10.3969/j.issn.1674-7968.2024.04.011
  研究论文与报告 本期目录 | 过刊浏览 | 高级检索 |
贝莱斯芽胞杆菌YFB3-1对辣椒疫霉的抑菌效果及其抑菌物质
穆晓清1, 鲁耀雄1,2,*, 戴良英1, 黄国林3, 陈薇4, 张榉邺4, 韦晨曦4
1 湖南农业大学 植物保护学院,长沙 410128;
2 湖南省农业科学院 农业环境生态研究所,长沙 410125;
3 湖南省农业科学院 园艺研究所,长沙 410125;
4 湖南农业大学 园艺学院,长沙 410128
Antibacterial Effect of Bacillus velezensis YFB3-1 on Phytophthora capsici and Its Antibacterial Substances
MU Xiao-Qing1, LU Yao-Xiong1,2,*, DAI Liang-Ying1, HUANG Guo-Lin3, CHEN Wei4, ZHANG Ju-Ye4, WEI Chen-Xi4
1 College of Plant Protection, Hunan Agricultural University, Changsha 410128, China;
2 Institute of Agri-environment and Ecology, Hunan Academy of Agricultural Sciences, Changsha 410125, China;
3 Horticultural Research Institute, Hunan Academy of Agricultural Sciences, Changsha 410125, China;
4 College of horticulture, Hunan Agricultural University, Changsha 410128, China
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摘要 辣椒疫病是由疫霉菌(Phytophthora capsic)侵染造成辣椒(Capsicum annuum)毁灭性损失的土传病害,严重威胁我国辣椒产业的可持续发展。为了获得安全高效、有效预防辣椒疫病的生防制剂,本研究选取从蚯蚓(Eisenia foetida)肠道细菌中分离鉴定的一株贝莱斯芽胞杆菌(Bacillus velezensis) YFB3-1为生防菌株,以辣椒疫霉菌为靶标菌,通过平板对峙法比较不同种类的芽胞杆菌对辣椒疫霉菌的抑菌效果,及YFB3-1对辣椒不同土传病原真菌的抑菌效果,并分析其主要的抑菌次级代谢物和生防机制。抑菌试验表明,贝莱斯芽胞杆菌YFB3-1对辣椒疫霉菌的抑制效果最好,抑菌率达85.21%,比枯草芽胞杆菌(Bacillus subtilis) GCK5-1对辣椒疫霉菌的抑菌率提高了19.8%,对辣椒不同土传病原真菌的抑菌效果较好,具有广谱抗辣椒土传病原真菌的特性。显微镜镜检表明,贝莱斯芽胞杆菌YFB3-1能够导致疫霉菌菌丝生长异常,多分枝或分枝杂乱,菌丝细胞不同程度地扭曲和膨大,甚至细胞壁破裂;液相色谱串联质谱(liquid chromatography tandem mass spectrometry, LC-MS)靶向代谢组测定结果显示,贝莱斯芽胞杆菌YFB3-1发酵液中的主要脂肽类化合物为表面活性素(surfactin)和丰原素(fengycin),在发酵3 d达最高含量,分别为1.19×10-3和1.14×10-3 mg/mL。本研究为辣椒疫病的绿色防控提供了优异的生防菌株,为进一步挖掘利用贝莱斯芽胞杆菌的次级代谢物质提供了参考依据。
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穆晓清
鲁耀雄
戴良英
黄国林
陈薇
张榉邺
韦晨曦
关键词 贝莱斯芽胞杆菌辣椒疫霉抑菌效果次级代谢物脂肽类化合物    
Abstract:Pepper phytophthora blight is a soil-borne disease caused by Phytophthora capsic, which causes devastating losses of pepper (Capsicum annuum) and seriously threatens the sustainable development of pepper industry in China. In order to obtain a safe, efficient and effective biocontrol agent for the prevention of pepper phytophthora blight, a strain of Bacillus velezensis YFB3-1 isolated and identified from the intestinal bacteria of earthworms (Eisenia foetida) was selected as a biocontrol strain, and Phytophthora capsici was used as the target strain. The antibacterial effects of different types of Bacillus on P. capsici were compared by plate confrontation method, and the antibacterial effects of YFB3-1 on different soil-borne pathogenic fungi of pepper were compared, and the main antibacterial secondary metabolites and biocontrol mechanisms were analyzed. The antibacterial test showed that B. velezensis YFB3-1 had the best inhibitory effect on P. capsici, and the inhibition rate was 85.21%, which was 19.8% higher than that of B. subtilis GCK5-1. It had good antibacterial effect on different soil-borne pathogenic fungi of pepper, and had the characteristics of broad-spectrum resistance to soil-borne pathogenic fungi of pepper. Microscopic examination showed that B. velezensis YFB3-1 could lead to abnormal mycelial growth, multi-branching or disordered branches, resulting in distortion and enlargement of mycelial cells to varying degrees, and even cell wall rupture. The results of liquid chromatography tandem mass spectrometry (LC-MS) targeted metabolomics showed that the main lipopeptide compounds in the fermentation broth of B. velezensis YFB3-1 were surfactin and fengycin, which reached the highest content at 3 d of fermentation, which were 1.19×10-3 and 1.14×10-3 mg/mL, respectively.This study provides an excellent biocontrol strain for the green prevention and control of pepper blight, and provides a reference for further excavation and utilization of secondary metabolites of B. velezensis.
Key wordsBacillus velezensis    Phytophthora capsici    Antibacterial effect    Secondary metabolite    Lipopeptide
收稿日期: 2023-08-02     
ZTFLH:  S476.8  
基金资助:湖南省重点研发计划(2021NK2008; 2022NK2062); 湖南省自然科学基金(2020JJ4393); 长沙市自然科学基金(kq2208105)
通讯作者: *luyaoxiong09@163.com   
引用本文:   
穆晓清, 鲁耀雄, 戴良英, 黄国林, 陈薇, 张榉邺, 韦晨曦. 贝莱斯芽胞杆菌YFB3-1对辣椒疫霉的抑菌效果及其抑菌物质[J]. 农业生物技术学报, 2024, 32(4): 859-872.
MU Xiao-Qing, LU Yao-Xiong, DAI Liang-Ying, HUANG Guo-Lin, CHEN Wei, ZHANG Ju-Ye, WEI Chen-Xi. Antibacterial Effect of Bacillus velezensis YFB3-1 on Phytophthora capsici and Its Antibacterial Substances. 农业生物技术学报, 2024, 32(4): 859-872.
链接本文:  
https://journal05.magtech.org.cn/Jwk_ny/CN/10.3969/j.issn.1674-7968.2024.04.011     或     https://journal05.magtech.org.cn/Jwk_ny/CN/Y2024/V32/I4/859
 
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