艾草HSP20基因家族鉴定及分析

doi:10.3969/j.issn.1674-7968.2025.11.005

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摘要热激蛋白20 (heat shock protein 20, HSP20)是一类小分子蛋白,在响应温度等非生物胁迫中发挥重要作用。鉴定并分析艾草(Artemisia argyi)中HSP20基因家族成员,有助于揭示艾草响应温度的调控机制。本研究利用生物信息学的方法鉴定出24个AaHSP20基因,并对其理化性质、进化关系、互作蛋白和表达模式等进行分析。结果表明,AaHSP20编码的蛋白氨基酸长度为155~215 aa,分子量为17.60~24.67 kD。大部分蛋白定位在细胞质。蛋白质二级结构主要由α-螺旋和无规则卷曲组成。24个AaHSP20基因不均匀地分布在10条染色体上。部分AaHSP20基因与拟南芥(Arabidopsis thaliana)和生菜(Lactuca sativa)中的同源基因存在共线性关系。系统进化分析表明,24个AaHSP20蛋白可划分为3类4个亚家族。AaHSP20蛋白均含有保守结构域和保守基序(motif 1和motif 2),且大部分成员基因结构中没有内含子。互作蛋白分析发现AaHSP20可能与其他小分子热激蛋白发生相互作用。qRT-PCR结果表明,5个候选AaHSP20基因在艾草不同组织中表达模式不同。AaHSP20-2、AaHSP20-7、AaHSP20-16和AaHSP20-21对高温与低温都敏感,AaHSP20-20只对高温敏感。本研究为进一步挖掘AaHSP20的功能及艾草抗性育种提供理论基础。

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	臧巧路
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关键词 ：艾草, 热激蛋白20(HSP20)基因家族, 生物信息学分析, 温度胁迫, 基因表达模式

Abstract：Heat shock protein 20 (HSP20) is a class of small molecular proteins that play critical roles in responding to temperature and other abiotic stresses. Identification and analysis of AaHSP20 gene family members in Artemisia argyi is helpful to reveal the regulatory mechanism response to temperature. 24 AaHSP20 genes were identified by using bioinformatics, and their physicochemical properties, evolutionary relationships, expression patterns, and so on were analyzed. The results showed that the encoded proteins ranged from 155 to 215 amino acids (aa) in length, the molecular weight was 17.60~24.67 kD, and most were predicted to be located in the cytoplasm. The secondary structures of AaHSP20 proteins were mainly composed of α-helix and random coils. The 24 AaHSP20 genes were unevenly distributed across 10 chromosomes. Some AaHSP20 genes had collinear relationships with homologs in Arabidopsis thaliana and Lactuca sativa. Phylogenetic analysis showed that the 24 AaHSP20 proteins could be divided into 3 classes and 4 subfamilies. All AaHSP20 proteins contained conserved domains and motifs (motif 1 and motif 2), and most genes lacked introns. Protein interaction analysis suggested AaHSP20 might interact with other small heat shock proteins. qRT-PCR analysis demonstrated that 5 candidate AaHSP20 genes exhibited distinct expression patterns in different tissues. AaHSP20-2, AaHSP20-7, AaHSP20-16, and AaHSP20-21 were sensitive to both high and low temperatures, while AaHSP20-20 was only sensitive to high temperature. These findings provide a theoretical foundation for elucidating the functions of AaHSP20 genes and improving resistance breeding of A. argyi.

Key words： Artemisia argyi Heat shock protein 20 (HSP20) gene family Bioinformatics analysis Temperature stress Gene expression pattern

收稿日期: 2024-12-02

中图分类号： S58

基金资助:山西农业大学博士科研启动项目(2022BQ13); 山西省基础研究计划(自由探索类)青年科学研究项目(202203021212454); 山西省中医药管理局科研课题(2023ZYYC2095; 2024ZYYB099); 山西省科技创新人才团队(202304051001018)

通讯作者: ^*^*mry1964@126.com

作者简介: ^*同等贡献作者

引用本文:

臧巧路, 郑晓美, 柳璐王梦苗如意. 艾草HSP20基因家族鉴定及分析[J]. 农业生物技术学报, 2025, 33(11): 2391-2403.
ZANG Qiao-Lu, ZHENG Xiao-Mei, LIU Lu WANG Meng MIAO Ru-Yi. Identification and Analysis of HSP20 Gene Family in Artemisia argyi. 农业生物技术学报, 2025, 33(11): 2391-2403.

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https://journal05.magtech.org.cn/Jwk_ny/CN/10.3969/j.issn.1674-7968.2025.11.005 或 https://journal05.magtech.org.cn/Jwk_ny/CN/Y2025/V33/I11/2391

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