Effects of Transport Stress on Apoptosis and Expression of Apoptosis-related Genes Bcl-2 and Bax in the Gastric Wall Tissues of Goat (Capra hircus)
HU Wei1,3, LIU Ben1,2,3,*, ZHENG Wen-Ya1,2,3, FANG Man-Xin1, HU Ying-Dong1, ZHANG Zhi-Tao1, ZHANG Wen-Yuan1, YE Tian1
1 College of Life Science and Resources and Environment, Yichun University, Yichun 336000, China; 2 Engineering Technology Research Center of Jiangxi Universities and Colleges for Selenium Agriculture, Yichun 336000, China; 3 Jiangxi Lvke Agriculture and Animal Husbandry Technology Co., Ltd., Yichun 336000, China
Abstract：With the development of goat (Capra hircus) breeding, transportation-induced health problems in goats cannot be ignored. The aim of this study was to investigate the effects of transport stress on cell apoptosis and the expression of apoptosis-related genes, such as B-cell lymphoma-2 (Bcl-2) and Bcl-2 associated X protein (Bax) in gastric wall tissue of Ganxi goat. Twelve healthy one-year-old Ganxi male goats were randomly divided into control group (n=4), 2 h transport group (n=4) and 6 h transport group (n=4). During the transportation process, food and water restrictions were carried out. After the transportation, tissues from stomach wall of the goats were collected and analyzed for relevant histology and quantification. Terminal dexynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL) results showed that the cell apoptotic rate of each stomach wall tissue in the 2 h transport group and the 6 h transport group was significantly increased (P<0.05) compared to the control group. Additionally, the cell apoptotic rate of other gastric wall tissues, except for omasum, was obviously higher in the 6 h transport group than in the 2 h transport group (P<0.05). Immunohistochemical results showed that Bax and Bcl-2 were mainly expressed in the mucosa epithelium of the anterior stomach (rumen, reticulum and omasum) and in the lamina propria of the abomasum. Bax expression in the 2 transport groups was clearly higher in the aforementioned areas of the stomach wall, with the decrease of Bcl-2 expression, but which was not obvious down-regulated in omasum. qPCR analysis revealed that the mRNA expression level of Bax was significantly up-regulated in other gastric wall tissues, except for omasum, in the 2 transport groups (P<0.05), whereas the Bcl-2 mRNA expression was significantly down-regulated (P<0.05). More importantly, Bax/Bcl-2 ratio in mRNA levels was significantly increased after transportation (P<0.05). Western blot results also showed that there was a similar pattern in the Bax and Bcl-2 protein expression to that of mRNA. These findings indicated that transport stress could lead to cell apoptosis in goat stomach wall tissue through regulating the expression of Bax and Bcl-2. This work may be able to serve as a theoretical foundation for investigating strategies to reduce the damage of goat organs and tissues caused by transport stress.
胡威, 刘犇, 郑文亚, 方满新, 胡迎东, 章志涛, 张雯苑, 叶恬. 运输应激对山羊胃壁组织细胞凋亡及凋亡相关基因Bcl-2和Bax表达的影响[J]. 农业生物技术学报, 2024, 32(2): 367-378.
HU Wei, LIU Ben, ZHENG Wen-Ya, FANG Man-Xin, HU Ying-Dong, ZHANG Zhi-Tao, ZHANG Wen-Yuan, YE Tian. Effects of Transport Stress on Apoptosis and Expression of Apoptosis-related Genes Bcl-2 and Bax in the Gastric Wall Tissues of Goat (Capra hircus). 农业生物技术学报, 2024, 32(2): 367-378.
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