Genetic Variation Analysis of Exon3 of MHC Gene and Its Correlation with Growth Performance in Two Goat (Capra hircus) Breeds
SUN Wang-Bin1,*, WANG Wei-Ping1, HUANG Yan2, SONG Xiao-Yue1, ZHANG Di1, SHANG Jia-Le1, ZHANGSUN Wei-Guang1, LI Zeng-Hui1
1 Shaanxi Province Engineering & Technology Research Center of Shanbei Cashmere Goats, Yulin University, Yulin 719000, China; 2 Yuyang District Animal Disease Prevention and Control Center, Yulin 719000, China
Abstract：Shanbei white cashmere goat (Capra hircus) and Ziwuling black goat are the main goats in the northern of Shaanxi, but their growth performance needs to be improved. Especially, the population size of the latter decreased sharply, so it is urgent to determine the genetic variation of its population. Genetic variation of major histocompatibility complex (MHC) genes can be used to evaluate population genetic diversity, and MHC genes are also associated with body size traits of domestic animals. The aim of this study was to investigate the polymorphism of major histocompatibility complex (MHC) gene exon3 in Shanbei white cashmere goat and Ziwuling black goat, and to evaluate the population development trend of Ziwuling black goat, and to
explore the correlation between MHC gene and growth performance or cashmere traits in Shanbei white
cashmere goats. In this study, the genetic variation of exon3 of MHC gene of 600 Shanbei white cashmere
goats and 30 Ziwuling black goats was analyzed by using high-throughput sequencing method, and the
correlation between MHC gene and growth performance was studied. Exon3 sequences of 2 MHC genes were
obtained, including 4 DQA1 alleles and 22 DQB2 alleles. The heterozygosity and polymorphism of Ziwuling
black goat were low, and the population development trend was not optimistic, so effective protection
measures should be taken. Moreover, Shanbei white cashmere goats had higher polymorphism, the BB
genotype of DQA1 gene was significantly higher than the other 2 genotypes in terms of height at hip cross and
wool length (P=0.035, P=0.021), and the AA genotype had the smallest cashmere fineness (P=0.003). These
alleles could be used as an effective molecular marker for growth performance improvement and ultrafine
cashmere goat breeding, respectively. The results of this study are helpful to promote molecular markerassisted
selection breeding of Shanbei white cashmere goat, and the protection, development and utilization of
Ziwuling black goat germplasm resources.
孙旺斌, 王伟萍, 黄艳, 宋晓越, 张迪, 尚佳乐, 长孙伟光, 李增辉. 两个山羊品种MHC基因外显子3的遗传变异分析及与生长性能的相关性[J]. 农业生物技术学报, 2022, 30(5): 935-943.
SUN Wang-Bin, WANG Wei-Ping, HUANG Yan, SONG Xiao-Yue, ZHANG Di, SHANG Jia-Le, ZHANGSUN Wei-Guang, LI Zeng-Hui. Genetic Variation Analysis of Exon3 of MHC Gene and Its Correlation with Growth Performance in Two Goat (Capra hircus) Breeds. 农业生物技术学报, 2022, 30(5): 935-943.
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