Sequence Variation and Tissue Expression Characteristics of Wnt5A Gene in Goat (Capra hircus)
LI Shao-Bin, ZHANG Rui-Guo, HE Zhao-Hua,ZHAO Peng-Fei, ZHAO Fang-Fang,WANG Ji-Qing,LIU Xiu,HU Jiang,LUO Yu-Zhu
College of Animal Science and Technology/Gansu Key Laboratory of Herbivorous Animal Biotechnology, Gansu Agricultural University, Lanzhou 730070, China
Abstract：The quality and yield of cashmere determine the economic value of cashmere goats (Capra hircus), and hair follicle is the direct tissue to regulate the growth and properties of cashmere. The properties of cashmere can be improved by regulating the development of hair follicle. Wingless-type mouse mammary tumor virus integration site family, member 5A (Wnt5A) gene is an key member in the signaling pathway relates with hair follicle development and play a key role in hair follicle development, but its research in goat is still very limited. In order to well know the molecular genetic characteristics of goat Wnt5A, the variation of this gene was detected by combining PCR-single strand conformation polymorphism (PCR-SSCP) and sequencing in Zhongwei goats, Chaidamu Cashmere goats and Longdong Cashmere goats. The tissue expression characteristics of Wnt5a in Liaoning Cashmere goats and Longdong Cashmere goats were analyzed by qPCR. The results showed that, in the 3 populations, there were 2 mutation sites in the Wnt5A exon 4 amplification region, which were c. 334c>A and c. 213a>G, respectively. Three alleles (A, B and C) were formed and showed five genotypes (AA, AB, AC, BB and BC). In Chaidamu cashmere goat and Longdong cashmere goat, A was the dominant allele, AB was the dominant genotype, while B in Zhongwei goat was the dominant allele and BB was the dominant genotype. Two mutation sites c. 685-5 C > T and c. 685-55 G>A were detected in Wnt5A exon 6, forming 2 alleles D and E, forming 3 genotypes DD, DE and EE. E was the dominant allele and DE was the dominant genotype in the 3 populations. Six haplotypes of H1~H6 were constituted by 2 amplified regions, and the most common one was H2. Wnt5A gene was expressed in all these 7 tissues of Liaoning Cashmere goat and Longdong Cashmere goat, with differences between breeds. At the same time, the expression difference in skin was significant, which speculated that resulted from the correlation between Wnt5A gene expression and the cashmere traits. By comparing the variation and expression characteristics of Wnt5A gene in different goat breeds, the molecular genetic characteristics of the gene were analyzed, which provided basic data for enriching the content of goat genome and the development and utilization of the gene.
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