Genome RNA was extracted from mesentery adipose of pig (Duroc Landrace Yorkshire) and lipoprotein lipase (LPL) mRNA was amplified using RT-PCR. A DNA fragment about 689 bp in length was obtained and the PCR product was cloned into pGEM-T vector. The LPL gene was isolated and sequenced from the screened positive clones. Result of sequence analysis showed that this fragment was the partial sequence of LPL cDNA and coded 279 amino acid residues. The gene homology of the obtained fragment compared with that of reported LPL gene sequence in adipose of porcine was up to 98%, and amino acid homology was 98.94%. Based on the LPL gene clone, an optimal semi-quantitative RT-PCR method was successfully constructed. Using β-actin as inner control, the difference of LPL gene expression at different avoirdupois of swine was researched, which increased from new born to 30 kg, decreased from 30 to 50 kg and rose again at the stage of 50 to 90 kg.