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| SCAR Molecular Markers of the B Biotype and Two Non-B Populations of the Whitefly, Bemisia tabaci (Hemiptera: Aleyrodidae) |
| ZANG Lian-sheng;IANG Tong;U Jing;IU Shu-sheng;HANG You-jun |
| Institute of Applied Entomology, Zhejiang University, Hangzhou 310029, China |
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Abstract RAPD was performed with random primer H16 for B biotype and two non-B populations of Bemisia tabaci collected from Zhejiang, China. The results revealed that the specific sequence fragments containing 446, 390 and 1317 nt were amplified for the B biotype, non-B ZHJ-1 and ZHJ-2 populations, respectively. The three specific fragments were cloned and sequenced, and three pairs of SCAR primers were designed according to the sequences. With improvement of the conditions of PCR, the specific fragment of B biotype, non-B ZHJ-1 and non-B ZHJ-2 populations, namely, 439, 366 and 1238 nt, respectively, could be amplified with the SCAR primer of the corresponding population, while the specific fragments of the other populations of B. tabaci or Trialeurodes vaporariorum could not be amplified.
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Received: 11 April 2005
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Corresponding Authors:
LIU Shu-sheng
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