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| Analysis and Sequencing of the Capsid Protein Gene of Rabbit hemorrhagic disease virus (RHDV) JX/97 Strain |
| LIU Guang-qing;NI Zheng;ZHANG Yu-ying;YUN Tao;LIANG Hua-li;HUA Jiong-gang; LI Shuang-mao |
Key Laboratory of Virology & Biotechnology, Ministry of Agriculture, Zhejiang
Academy of Agriculture Sciences, Hangzhou 310021, China |
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Abstract The capsid protein gene of Rabbit hemorrhagic disease virus (RHDV) JX/97 strain was cloned and sequenced .The results showed that the nucleotide sequence of the gene was 1 739 nt in length encoding a protein of 580 aa. Variation analysis of the capsid protein gene of JX/97 strains and other 21 published RHDV isolates showed that RHDV had a high degree of conservation, the homology of the amino acids among different isolates exceeded 90%. The results of computer analysis showed that the antigenic region hadn’t changed remarkably in past 20 years. The results of phylogenetic tree analysis showed that all the reference RHDV isolates could be divided into 4 genetic groups and the distant between them appeared to have evolved to different extents, that means during adapting to the special environment, RHDV may form its only biology characteristic gradually.
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Received: 30 March 2005
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Corresponding Authors:
LIU Guang-qing
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ZHAO Zhong-Hai, ZHOU Di, LI Hui, YI Heng-Jie, LONG Qing-Meng, SHI Xin-Cai, BU Xiao-Yan, Chen Lin, YANG Hua-Ting, LI Xing-Cai. Genetic Diversity and Origin of Guizhou Pig (Sus scrofa domesticus) Breeds Analyzed by mtDNA D-loop Region[J]. 农业生物技术学报, 2019, 27(7): 1206-1214. |
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