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| Sequence Analysis of the σC Gene of Muscovy duck reovirus ZJ99 Strain and Its Expression in Escherichia coli |
| YU Xu-ping;HE Shi-cheng;ZHU Jun-li;JIN Jun-jie;ZHENG Xin-tian |
| Animal Science College, Zhejiang University, Hangzhou 310029, China |
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Abstract The σC gene (GenBank: AY619690) of Muscovy duck reovirus (mDRV) ZJ99 strain was amplified and sequenced with RT-PCR. BLASTn analysis results showed that the σC gene of mDRV ZJ99 had 98% homology to mDRV MW9710 isolated in Fujian Province of China, 92% and 93% homology to mDRV 89026 and mDRV 89330 reported in France. BLASTp analysis showed that the deduced amino acid sequence of ZJ99 had 94%, 89%, 89% identities and 95％, 92％, 93％ similarities to that of MW9710, 89026 and 89330, respectively. The σC gene was further subcloned into pET-28a and transformed into Escherichia coli BL21(DE3). SDS-PAGE analysis showed that the σC protein was successfully expressed in E. coli, which existed as inclusion body and accumulated up to 22.9% of total cellular proteins.
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Received: 21 February 2005
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Corresponding Authors:
YU Xu-ping
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