Rapid Determination of Ploidy Level of Chromosome in Tobacco(Nicotiana tabacum)
ZHU Hui-qin, ZHANG Xian-yin, XUE Qing-zhong
1. College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029,China; 2. College of Agriculture and Stockbreeding, Qinghai University, Xining 810003, China
Abstract Abstract: The ploidy level of 763 anthers deirived from both crosses (G28×NC2326 and K326×Coker176) of tobbaco (Nicotiana tabacum) were determined. The result of identification by counting the number of stoma chloroplast in guard cell was consistent with those observations of flower and seed fertillity of the plants, with the average accurate rate of 93.52%, showing that measurement of the number of stoma chloroplast in guard cell could be considered as a fast and accurate method to determine haploid, diploid or poly-ploid of tobacco in the seedling stage. In order to reduce the waste of haploid plants and speed up establishment DH population, it was feasible to select out undoubted haploid plants with <14 chloroplast number in stoma treated with colchicine when young plantlets just transplanted in the field.
