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| Identification of Bovine and Sheep Derived Materials in Feedstuff by Semi-nested PCR |
| WEN Wei-gang;CUI Jun-xia;ZHAO Xiu-ling |
| Ningbo Entry-Exit Inspection and Quarantine Bureau, Ningbo 315012，China |
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Abstract Augment identification of bovine and sheep derived materials in feedstuff is essential for effective control transmission of bovine spongiform encephalopathy and scrapie. This study described a semi-nested PCR assay for the detection of bovine and sheep materials in feedstuff. Bovine- or sheep-specific semi-nested PCR primer had been designed for this purpose based on their published species-specific gene and primer’s sequences. The results of semi-nested PCR showed that 247 and 214 bp bands were obtained from the bovine and sheep materials respectively, the detection limitation was 0.00001%～0.0001% for bovine and sheep derived materials in feedstuff and 10-6 ng～10-5 ng for bovine and sheep genomic DNA respectively, 103 and 105 times higher than that of the detection limitation of PCR. Therefore, the rapid, sensitive and stable semi-nested PCR are the effective methods for detecting trace bovine and sheep derived materials in feedstuff.
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Received: 20 March 2005
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Corresponding Authors:
WEN Wei-gang
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JIN Ya-Qi, YU Er-Meng, ZHANG Kai, LI Zhi-Fei, WANG Guang-Jun, XIE Jun, YU De-Guang, SUN Jin-Hui, WEI Dong, GONG Wang-Bao, TIAN Jing-Jing. Effects of Three Feeds on Serum Enzyme Activity, Intestinal Structure and Bacterial Flora of Ctenopharyngodon idellus[J]. 农业生物技术学报, 2019, 27(9): 1652-1663. |
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