Differential Display and Sequence Analysis of Senescence-associated Genes in Cut Roses
Bai Shangyi1 Liu Qinglin1* Ouyang Qing2 Cai Wenqi2
(1. Department of Ornamental Horticulture and Landscape Architecture, China Agricultural University, Beijing 100094, China; 2. Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China )
Abstract：Abstract: Using DDRT-PCR, twenty-two cDNA specific fragments were amplified from petal total RNA of Rosa cv. Pavarotti with longer vase life. Nine of those cDNA specific fragments were cloned, which were b21 , b32（b33）, b43 , b51（b52 , b814）, b618（b101 , b108 , b813）, b624 , b131 , b134 and b186 respectively. Sequences blasted in GenBank showed that b618 and b624 derived from recombination plasmid b6 were two different sequences. b618 , which had 439 bp and 137 deduced amino acid, was 71%~79% homologous to that of corresponding encoding regions of chalcone isomerase(CHI), such as Arabisopsis thaliana, Citrus sinensis, Raphanus sativus, Malus sp., Vitis vinifera and Elaeagnus umbellata . While b624 , which had 440 bp and 134 deduced amino acid,was 86%~90% identical to the cholinephosphate cytidylyltransferase (CPCT) with Arabidopsis thaliana, Pisum sativum and Brassica napus. RT-PCR showed b618 and b624 were positive results. CHI was an enzyme involved in flavonoid biosynthesis pathway, while CPCT is responsible to CDP-choline pathway, both were related to the senescence process.
