Abstract：Abstract:Three kinds of T-A cloning methods were tested to compare the efficiency of four different length PCR products. The results indicated that high cloning efficiency was obtained using common T-A cloning method for short PCR product(Length is about 500 bp), the cloning efficiency was improved using temperature cycle T-A cloning method for moderate PCR product(Length is about 3 000 bp) and the cloning efficiency was increased using two-step recombinant T-A cloning method for long PCR product(Length is about 6 000 bp).
