Cloning and Expression of Self-incompatibility Related S-RNase Gene in Apricot
Qi Jie1,2 Gu Manru1 Shu Huairui1
(1. College of Horticulture, Shandong Agriculture University,Taian 271018,China; 2. Department of Marine Biology, Ocean University of Qingdao,Qingdao 266003,China)
Abstract：Abstract:Three cDNAs named PA1, PA2 and PA3 were isolated from self-incompatibility cultivars of Badanshui, Hongyu and self-compatibility Katy apricot(Prunus aremeniaca ). They encode 576 , 601 and 591 bp，respectively.There was 80.6% similarity among their amino acid sequences. The comparison of amino acid sequences of Rosaceae S-RNase showed highly divergent, and intra-subfamilial similaritiy was higher than that of inter-subfamilial one. Fourteen sites were perfectly conserved among encoded 187 amino acid residues , and in hyperaviable region(RHV) two amino acid residues (H, M) in PA1 and three amino acid residues(N, S, A) in PA3 were different from other S-RNase, which indicated the different amino acid residues might play a role of S-allele gene special recognition. From the phylogentic tree about Rosaceae, Solanaceous and some S-like RNase, apple and pear belong to Maloideae, but cherry, almond and apricot belong to Amygdaloideae, and three sequences were identified to be homology to S-RNase not to S-like RNase from other families. Northern blotting analyses indicated that PA3 was expressed predominatly in pistils of Kate apricot, which molecular weight was 0.9 kb, but not expressed in root, stem and leaf of Katy apricot. According to the sequence analyses and blast in GenBank, the result showed the obtained three genes were expression genes of S-RNase.
