Abstract：Abstract Festuca arundinacea is a widely cultivated forage in the world with excellent stress and disease resistance. Qiancao No.2 was cultivated by Guizhou Institute of Prataculture, which has two different strains (broad-leaved and fine-leaved) with obvious differences in the leaf shape. In order to recover the mechanism of leaf formation and resistance of Qiancao No.2, in this study, a differential display reverse transcription PCR (DDRT-PCR) was performed to screen the differential expression genes between the broad-leaved and fine-leaved strains. Finally, 50 differential bands were separated and recovered. The DNA fragments were cloned and sequenced. Most of different expressed genes were enriched into photosynthesis, growth regulation and stress resistance. After further verification by qRT-PCR, it was found that only the genes of 18S ribosome were up-regulated in broad-leaved, and the rest of them were up-regulated in fine-leaved. These results suggested that plants with large leaves usually allocated more biomass to the cell wall while to enhance the toughness of leaves, and accumulated more photosynthetic products to prepare for the growth and development, this situation would lead to lower photosynthetic efficiency in unit area of leaves. However, plants with narrow leaves allocated more organic in chloroplast which made them had higher photosynthetic capacity to accelerate growth, and with strong competition ability in natural environment. In particular, the growth of aboveground parts made them with strong competition ability in natural environment. This study provides a theoretical foundation for the further research on the functional genes of leaf morphogenesis, gene expression and the stress resistance.
[1] 刘晓，岳明，任毅.独叶草叶片性状表型多样性研究[J].西北植物学报, 2011, 31(5):950-957
[2] 邓晓青，姚晓华，吴昆仑，等.青稞LTP蛋白基因bltl4.2的克隆及其在低温下的表达[J].中国农业大学学报, 2012, 17(2):18-24
[3] 陈菲，杨郁文，何冰，等.棉花P-type ATPases基因的克隆及表达分析[J].江苏农业学报, 2011, 27(6):1192-1197
[4] 杜佳，杨静莉，李成浩.林木金属硫蛋白研究进展[J].植物生理学报, 2011, 47(8):759-764
[5] 牛熙，王嘉福，陈超.两个高羊茅品种苗期抗旱性的比较[J].山地农业生物学报, 2012, 31(2):132-136
[6] 李志能，黄文俊，张佳琪，等.异硫氰酸胍法快速提取二球悬铃木组织总RNA的研究[J].植物科学学报, 2007, 25(3):266-269
[7] Liang P, Pardee A B.Differential Display of Eukaryotic Messenger RNA by Means of the Polymerase Chain Reaction[J].Science, 1992, 257(5072):967-971
[8] Sugiura M, Iwai E, Hayashi H, et al.Differences in the interactions between the subunits of photosystem II dependent on D1 protein variants in the thermophilic cyanobacterium Thermosynechococcus elongatus[J].Journal of Biological Chemistry, 2010, 285(39):30008-30018
[9] Maldonado A M, Doerner P, Dixon R A, et al.A putative lipid transfer protein involved in systemic resistance signalling in Arabidopsis[J].Nature, 2002, 419(6905):399-403
[10] Babanova S, Matanovic I, Chavez M S, et al.Role of Quinones in Electron Transfer of PQQ–Glucose Dehydrogenase Anodes—Mediation or Orientation Effect[J].Journal of the American Chemical Society, 2015, 137(24):7754-7762
[11] Newman D K, Kolter R.A role for excreted quinones in extracellular electron transfer[J].Nature, 2000, 405(6782):94-
[12] Samuilov V D, Kiselevsky D B.Effect of cationic plastoquinone SkQ1 on electron transfer reactions in chloroplasts and mitochondria from pea seedlings[J].Biochemistry, 2015, 80(4):417-423
[13] Bald D.ATP Synthase: Structure, Function and Regulation of a Complex Machine[M]. 2011: 239-261.
[14]Feniouk B A, Suzuki T, Yoshida M.The role of subunit epsilon in the catalysis and regulation of FOF1-ATP synthase[J].Biochimica Et Biophysica Acta, 2006, 5(6):326-338
[15] Zhang X, Liu S, Takano T.Overexpression of a mitochondrial ATP synthase small subunit gene (AtMtATP6) confers tolerance to several abiotic stresses in Saccharomyces cerevisiae and Arabidopsis thaliana[J].Biotechnology Letters, 2008, 30(7):1289-1294
