Abstract：In order to research the synergistic effect of glucose of insulin on lipogenesis in goose liver, we measured the effect of glucose of insulin on triglycerides (TG) accumulation, enzyme activity and mRNA expression of fatty acid synthase (FAS) in primary hepatocytes of Sichuan White Goose and Landes Goose. Our results were as follows: Glucose alone could induce the TG accumulation, enzyme activity and gene expression of FAS in goose primary hepatocytes of both breeds, and the induction effect was more evident when glucose of insulin cultured together. In addition, there was difference of effect of insulin and glucose on the TG accumulation, enzyme activity and gene expression of FAS in hepatocytes between the two breeds. Conclusions: Glucose alone could induce the increase of mRNA expression and enzyme activity of FAS, then resulted in the TG accumulation in hepatocyytes. Meanwhile, the induction was more evident when glucose and insulin cultured together. In addition, there was breed difference of synergistic effect of glucose of insulin on lipogenesis.
