Effects of L-NAME and L-Arg on Meiotic Maturation of Mouse Oocyte in Vivo
Bu Shumin1,2 Xia Guoliang1** Wang Songbo1 Wang Jianhong1,3
(1. College of Biological Sciences, China Agricultural Univesity, Beijing 100094, China; 2. College of Life Science and Technology, Shanxi University, Taiyuan 030006, China; 3. College of life and environment Sciences, XinJiang Normal University, Urumqi 830054, China)
Abstract：Abstract: The effect of nitric oxide (NO)on meiotic maturation of mouse oocyte in vivo was investigated by injecting L-NAME alone or co-injecting L-NAME and L-Arg intraperitoneally. The results showed that: (1) 5, 10 and 20 mg/kg bw (body weight) of L-NAME inhibited significantly the extrusion of first polar body of mouse oocyte(P <0.01), especially 10 mg/kg bw. But they all had little impact on germinal vesicle breakdown (P >0.05). (2) The inhibitory effect of L-NAME (10 mg/kg bw) on the extrusion of first polar body of mouse oocyte could be reversed by 100 mg/kg bw or 200 mg/kg bw L-Arg.(3) The abnormality caused by L-NAME was related to the culture time, but L-NAME had no significant effect on viability of mouse oocytes. It could be concluded that NO was involved in regulation of mouse oocyte meiotic maturation in vivo.
卜淑敏1,2 夏国良1** 王松波1 汪建红1,3. NO合酶抑制剂 L-NAME和NO前体L-Arg 对小鼠体内卵母细胞成熟的影响 [J]. , 2003, 11(3): 291-294.
Bu Shumin1,2 Xia Guoliang1** Wang Songbo1 Wang Jianhong1,3. Effects of L-NAME and L-Arg on Meiotic Maturation of Mouse Oocyte in Vivo . , 2003, 11(3): 291-294.
