Cloning of germin Gene and Its Expression in Tobacco
Wang Bingshan1 Dou Daolong2 Zhang Meng3 Wang Zhixing1 Jia Shirong1
（1. Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 2. Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China; 3. Horticulture Department, Northwest Science and Technology University of Agriculture and Forestry, Yangling 712100, China）
Abstract：Abstract: Oxalic acid was produced by several plant pathogenic fungi and thought to ploy a primary role in the pathogenicity of fungal species, including the wide host-range pathogen Sclerotinia sclerotiorum. In the current work, a germin gene from wheat (Triticum aestivum )was cloned. A plant expression vector containing the germin gene was constructed by CaMV 35S promoter, which was used for Agrobacterium tumefaciens-mediated transformation of tobacco(Nicotiana tabacum ). Southern blotting assay indicated that the target gene was integrated into tobacco genome. Enzymatic activity assay of oxalate oxidase in transgenic tobacco showed that the germin gene was expressed, which resulted in the formation of homohexamer with enzymatic activity correctly, and the transgenic plants were tolerant of the exogenously supplied oxalic acid.
