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2025年7月30日 星期三
  研究论文 本期目录 | 过刊浏览 | 高级检索 |
germin基因的克隆及其在烟草中的表达
王冰山1 窦道龙2 张 猛3 王志兴1 贾士荣1
(1. 中国农业科学院生物技术研究所, 北京 100081; 2. 中国科学院植物研究所, 北京 100093;
3. 西北农林科技大学园艺系,杨凌 712100)
Cloning of germin  Gene and Its Expression in Tobacco
Wang Bingshan1 Dou Daolong2 Zhang Meng3 Wang Zhixing1 Jia Shirong1
(1. Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing  100081, China;
2. Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China;
3. Horticulture Department, Northwest Science and Technology University of Agriculture and Forestry, Yangling 712100, China)
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摘要 摘要:克隆了具有草酸氧化酶活性的小麦(Triticum aestivum )Germin蛋白的基因,构建了植物表达载体并用根癌农杆菌(Agrobacterium tumefaciens )介导法转化烟草。Southern杂交和酶活性检测表明,germin基因在转基因烟草(Nicotiana tabacum )中得到整合、表达,并正确组合成具有草酸氧化酶活性的同源六聚体蛋白。离体实验表明,外源germin基因的表达提高了转基因烟草离体叶片对草酸的耐受性。
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王冰山
窦道龙
张猛
王志兴
贾士荣
关键词 关键词:germin 基因转基因烟草草酸    
AbstractAbstract: Oxalic acid was produced by several plant pathogenic fungi and thought to ploy a primary role in the pathogenicity of fungal species, including the wide host-range pathogen Sclerotinia sclerotiorum. In the current work, a germin gene from wheat (Triticum aestivum )was cloned. A plant expression vector containing the germin gene was constructed by CaMV 35S promoter, which was used for Agrobacterium tumefaciens-mediated transformation of tobacco(Nicotiana tabacum ). Southern blotting assay indicated that the target gene was integrated into tobacco genome. Enzymatic activity assay of oxalate oxidase in transgenic tobacco showed that the germin gene was expressed, which resulted in the formation of homohexamer with enzymatic activity correctly, and the transgenic plants were tolerant of the exogenously supplied oxalic acid.
Key wordsKey words: germin gene;transgenic tobacco;oxalic acid
收稿日期: 1900-01-01     
通讯作者: 王冰山1    
引用本文:   
王冰山1 窦道龙2 张 猛3 王志兴1 贾士荣1 . germin基因的克隆及其在烟草中的表达[J]. , 2003, 11(3): 233-235.
Wang Bingshan1 Dou Daolong2 Zhang Meng3 Wang Zhixing1 Jia Shirong1 . Cloning of germin  Gene and Its Expression in Tobacco. , 2003, 11(3): 233-235.
链接本文:  
http://journal05.magtech.org.cn/Jwk_ny/CN/      或     http://journal05.magtech.org.cn/Jwk_ny/CN/Y2003/V11/I3/233
 
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