Overexpression of 5-enolpyruvylshikimate-3-phosphate Synthase Gene (CP4EPSPS) under the Control of Leafy Promoter Enhances Glyphosate Tolerance of Budlet
Abstract：causes abnormality of plant growth. To reduce the burden of plant metabolism and to prevent energy waste, we have used a Leafy promoter from genome of Arabidopsis thaliana instead of CaMV35S promoter to govern the expression of a modified 5-enolpyruvylshikimate-3-phosphate synthase gene (CP4EPSPS) with addition of the transit peptide-coding sequence of rubisco small ribosome subunit. Meanwhile, the Leafy promoter was used to regulate gus, a reporter gene. Two plant expression vectors, p3300-Leafy-gus and p3300-Leafy-CP4EPSPS were constructed and used to transform tobacco W38 through Agrobacterium-mediated procedure. Under these conditions, stable expression of the CP4EPSPS and gus genes was only observed in stem apex and extremely young apical leaves of transgenic tobaccos. The gus activity was not detectable in mature leaves, stems or roots. It is demonstrated that the budlet of transgenic tobaccos shows tolerance to herbicide glyphosate
