DNA Polymorphism Analysis and a Sequence-characterized Amplified Region (SCAR) Markers of Streptomyces roseoflavus Menmyco-93-63 and Its Mutant Strains
Abstract：Streptomyces roseoflavus Men-myco-93-63 isolated from potato scab decline soil is an antagonistic strain. The strain and its fermentation can inhibit many pathogenic fungi and control the related important plant disease such as Verticillium wilt of cotton, cumber powdery mildew, and so on. The genetic polymorphism of Men-myco-93-63 with its eight antibiotic blocked mutants was analyzed using amplified fragment length polymorphism(AFLP) and 3 142 AFLP bands were amplified using 38 primer pairs, and 2 362(75.2 %) bands of them were polymorphic. Three of the 96 pairs of primers E-CA/M-GA, E-GA/M-AC and E-GA/M-AG were selected for AFLP. The bands with 254, 312 and 209 bp, which amplified by the three pairs of primers respectively, were present only in Men-myco-93-63 and named as EM254, EM312 and EM209. After recovery, cloning and sequencing，the three specific AFLP fragments were successfully converted into single locus PCR marker of a sequence-characterized amplified region (SCAR) which could be more easily to be used to detect mutants of Men-myco-93-63 at the molecular level.
