An Efficient Identification of Eurasian Grape(Vitis vinifera L.) Cultivars with a Strategy of Manual Cultivar Identification Diagram (MCID) Method Using DNA Markers
Abstract：Because of the high similarity in the botanical characters of the Eurasian grape varieties, it is difficult to distinguish or identify a large number of varieties using the traditional single morphological methods, especially the strains and elite cultivars derived from a small number of parents that show similar traits. In this study, random primers with the composition of 11 bases were selected for RAPD amplification of the Eurasian grape (Vitis vinifera L.) varieties, and corresponding map relationships were bulit according to the specific bands, thus plant species were identified through drawing the diagram manually (manual cultivar identification diagram, MCID), which distinguished different varieties efficiently and quickly. This method was convenient, fast and accurate, and 191 grape varieties could be distinguished using 7 times PCR at the molecular level. Compared to the clustering tree, the manual CID could be easy to read and have more practical use. In other words, the primers for distinguishing between any two varieties could be found according to the MCID chart. This method can achieve early identification of seedlings, and has a wide range of versatility in the other species. Furthermore, it will have a positive meaning in the identification of grape germplasm resources and promote sustainable development of wine industry
