Abstract：Rye (Secale cereale) with multi-spikelet and resistant gene to powdery mildew, is beneficial for wheat genetic improvement. Wheat germplasm N9436-1 is bred from the progeny of Austria rye × common wheat （Triticum aestivum） Shaanmai 611. In the present study, cytology, Genomic in situ hybridization (GISH), Giemsa C-banding, sequence characterized amplified region (SCAR) and A-PAGE were used to detect rye chromatin of N9436-1. The result showed that the morphology and cytology of N9436-1 was stable with chromosome configuration of 2n=44=22Ⅱ. N9436-1, with multi-spikelet, was immune to powdery mildew. GISH and C-banding showed that N9436-1 was wheat-rye 1R disomic additional line. SCAR and APAGE analysis further displayed that the line N9436-1 had the genetic material of rye. Pm8 and Pm17 of 1R chromosome were not resistant to powdery mildew, while N9436-1 was resistant to it. So there is probably a new gene on 1R chromosome of the line N9436 resisting to powdery mildew and can be used as resistant resource of powdery mildew for wheat breeding.
