Abstract： SSR analysis was performed based on 7 wheat (Triticum aestivum) near iso-genic lines (NILs) with Lr genes located on chromosome 2D including two multiple alleles of Lr2c, the susceptible control Thatcher, and a population of 215 F2 plants derived from TcLr2c × Thatcher, respectively to identify the SSR marker linked to wheat leaf rust resistance gene Lr2c. Twenty-nine SSR primer pairs on wheat 2D chromosome were screened and four of them could generate polymorphisms to the other near isogenic lines. Result showed that Xgwm261 and Xgwm296 were tightly linked to the gene Lr2c with a distance of 1.9 and 3.6 cM respectively using test and verify of the population of 215 F2 derived from TcLr2c × Thatcher, which could be used in molecular assisted breeding against wheat leaf rust.
