Identification of FLOWERING LOCUS C(FLC) Gene in 2# Chinese Cabbage(Brassica rapa ssp. pekinensis)-cabbage(B. oleracea var. capitata) Monosomic Alien Addition Line
摘要Flowering time is an important trait in Brassica, and FLOWERING LOCUS C(FLCs) are key genes to flowering. In this paper, FLCs specific primers, polymorphic not only in Brassica rapa ssp. pekinensis and B. oleracea var. capitata, respectively, but also between B. rapa ssp. pekinensis and B. oleracea var. capitata, using DNA samples from 4 different type of B. oleracea var. capitata and 4 different types of B. rapa ssp. pekinensis individual plants, were screened. The 4 specific primers were obtained for genotyping of BrFLC1, BrFLC2, BrFLC3, BrFLC5, BoFLC1, BoFLC2, BoFLC3 and BoFLC5. Forward primers were designed on polymorphic region of exon 4, and reverse primers were designed on conserved region of exon 7. The corresponding FLCs between B. rapa ssp. pekinensis and B. oleracea var. capitata could be identified by 1 pair of primers. The FLCs from B. rapa ssp. pekinensis and B. oleracea var. capitata in 1 cell could be differentiated easily and quickly using 4 specific pairs of primers. The same FLCs specific bands, amplified by 4 specific primers, appeared in sesquidiploids hybrid (AAC) drived from tetraploid B. rapa ssp. pekinensis (AAAA) and diploid B. oleracea var. capitata (CC), as well as in B. rapa ssp. pekinensis and B. oleracea var. capitata. In the B. rapa ssp. pekinensis monosomic alien addition line, which introduced chromosome 2 from B. oleracea var. capitata, besides the 4 BrFLCs genes, only BoFLC3 existed in the monosomic alien addition line, detected by 4 specific primers. The results provide a new way to identify BoFLCs in the background of B. rapa ssp. pekinensis, and supply special materials for translocation lines added BoFLC3 under B. rapa ssp. pekinensis background.
Abstract：Flower time is important trait in Brassica, FLCs are key genes to flowering. Forward primers were designed on polymorphic region of exon 4, reverse primers were designed on conserved region of exon 7. Four Specific primers were developed for Genotyping of BrFLC1, BrFLC2, BrFLC3, BrFLC5, BoFLC1, BoFLC2, BoFLC3 and BoFLC5. FLCs were identified by above mentioned specific primers in the Chinese cabbage monosomic alien addition line which introduced chromosome 2 from cabbage, the results showed that besides the 4 BrFLCs, BoFLC3 was existing in the monosomic alien addition line. Which provide new way to identification of BoFLCs in the background of Chinese cabbage, and supply generation of Chinese cabbage translocation lines added BoFLC3 with special materials.
