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2025年7月30日 星期三
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利用复合杂交群体定位陆地棉产量性状QTL
张建
西南大学农学与生物科技学院
QTL Mapping of yield traits with composite cross population in upland cotton (Gossypium hirsutum L.)
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摘要 以共同亲本渝棉1号分别与中棉所35和贝尔斯诺杂交F1代,再次杂交得到复合杂交群体。利用6565对SSR引物对3亲本进行多态性引物筛选,获得92对多态性引物。以多态性引物检测复合杂交群体172个单株的标记基因型,共获得96个标记位点,其中4对引物产生两个位点。构建的遗传连锁图谱包括63个标记、19个连锁群,总长656.0 cM,标记间平均距离10.4 cM,覆盖棉花基因组14.8%。利用多QTL作图方法,以复合杂交群体F2株系的产量性状鉴定结果,检测到7个产量性状QTL,即1个单株铃数(BN)、2个单铃重(BW)、1个衣分(LP)、1个单株籽棉(SC)和2个单株皮棉(LC)。7个QTL分布于4条D基因组染色体,其中第19染色体分布4个QTL。同时,不同亲本之间存在产量性状QTL等位基因的差异。
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张建
关键词 陆地棉 复合杂交群体 遗传连锁图谱 产量QTL    
Abstract:A composite cross population was derived from the cross between (Yumian 1 × CRI 35) F1 and (Yumian 1 × Belshinuo) F1. A total of 6,565 primer pairs were used to screen the polymorphic primers between CRI 35, Yumian 1 and Belshinuo, and 92 polymorphic primer pairs were obtained. The polymorphic primer pairs were used to genotype the 172 individual plants of composite population and 96 loci were obtained. Four out of 92 primer pairs produced two loci. A genetic linkage map with 63 SSR loci and 19 linkage map was constructed, and the linkage map covered a whole length of 656 cM with an average interval of 10.41cM between two markers, accounting for 14.8% of the cotton genome. The yield traits of the 172 composite population F2 lines were used to detect QTL by multiple QTL mapping, and 7 QTL for five yield traits were identified, including one for boll number per plant (BN), two for boll weight (BW),one for lint percentage (LP), one for seed cotton yield per plant (SC), and two for lint cotton yield per plant (LC). Seven QTL were mapped on four D-genome chromosomes, and four QTL were mapped on chromosome 19. It was found that the different parents have different QTL alleles of yield trait.
Key wordsUpland cotton    Composite population    Genetic linkage map    Yield    QTL
收稿日期: 2009-04-30     
通讯作者: 张建   
引用本文:   
张建. 利用复合杂交群体定位陆地棉产量性状QTL[J]. , 2010, 18(3): 476-481.
链接本文:  
http://journal05.magtech.org.cn/Jwk_ny/CN/     或     http://journal05.magtech.org.cn/Jwk_ny/CN/Y2010/V18/I3/476
 
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