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2025年8月13日 星期三
  2017, Vol. 25 Issue (1): 43-49    
  研究论文与报告 本期目录 | 过刊浏览 | 高级检索 |
辣椒胞质雄性不育主效恢复性的QTL定位
魏兵强1,王兰兰2,张茹2,张建农3
1. 甘肃农业大学园艺学院
2. 甘肃省农业科学院蔬菜研究所
3. 甘肃农业大学农学院
Identification of Two Major Quantitative Trait Loci Restoring the Fertility of Cytoplasmic Male Sterility in Capsicum annuum
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摘要 辣椒(Capsicum annuum)胞质雄性不育可以被核内恢复基因恢复,然而人们对这些核内恢复基因的数量和作用方式仍然知之甚少。本研究利用QTL IciMapping 作图软件构建了一张辣椒种内分子遗传图谱,利用该分子遗传图谱,共鉴定到与辣椒胞质雄性不育恢复性相关的2个主效QTL和7个微效QTL,2个主效QTL的遗传率高达92.58%。第1个主效QTL(qIF-3-1)可解释表型变异的46.68%,显性效应和加性效应分别为1.28和0.84。另1个主效QTL(qIF-5-1)可解释表型变异的47.10%,显性效应高达1.76。7个微效QTLs中,3个主要起加性效应,4个起显性效应。该结果不仅为开发辣椒胞质雄性不育恢复性的分子标记提供了指导,而且有助于深入了解辣椒胞质雄性不育育性恢复的调控机制。
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魏兵强
王兰兰
张茹
张建农
关键词 辣椒胞质雄性不育育性恢复QTL    
Abstract:The fertility of cytoplasmic male sterility (CMS) can be restored by nuclear-encoded fertility restorer genes. However, the number and modifications of these restorer nucleor genes remain poorly understood. In this study, using QTL IciMapping software an intraspecific genetic linkage map of Capsicum annuum was constructed, and 2 major and 7 minor quantitative trait loci (QTLs) were identified, which acting as restorer-of-fertility (Rf) genes counteracting CMS. The heritability of the 2 major loci reached 92.58%. The first most important allele, qIF-3-1, contributed to fertility with a phenotypic variance explained (PVE) of 46.68%, with dominant and additive effects of 1.28 and 0.84, respectively. The other major allele contributing to fertility, qIF-5-1, was associated with a PVE of 47.10% and improved fertility with higher dominant effects of 1.76. Among 7 minor QTLs, 3 QTLs mainly acted as additive effect, while another 4 as dominant effect. These results are beneficial for our understanding of the mechanisms regulating the function of Rf genes. Moreover, our results provide guidance for selecting molecular markers in the development of marker-assisted selection (MAS) fertility restoration breeding schemes in peppers.
Key wordsCapsicum annuum    Cytoplasmic male sterility    Fertility restorer    Quantitative trait loci
收稿日期: 2016-06-08      出版日期: 2016-12-24
基金资助:马麝病毒性出血症的病原分离鉴定及其生物学特性研究
通讯作者: 张建农     E-mail: zjn0101@sina.com
引用本文:   
魏兵强 王兰兰 张茹 张建农. 辣椒胞质雄性不育主效恢复性的QTL定位[J]. , 2017, 25(1): 43-49.
链接本文:  
http://journal05.magtech.org.cn/Jwk_ny/CN/     或     http://journal05.magtech.org.cn/Jwk_ny/CN/Y2017/V25/I1/43
 
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