Abstract：Adult half-smooth tongue-sole (Cynoglossus semilaevis) differs widely in size between male and female, and sex control is an effective measure to improve economic profit for half-smooth tongue-sole aquaculture and the key to achieving sex control is to reveal the genetic mechanism of sex determination. The pattern of half-smooth tongue-sole's genetic sex determination is ZW type. This kind of type may exist multi-factor dose effect, that is, sex determination is not only related with sex chromosomes, but also autosomes may involve in the regulation of sex. Therefore, genetic sex is the result of sex chromosomes and autosomes’ synergistic effect. In this study, we tried to investigate the mechanism of half-smooth tongue-sole’s sex determination from the perspective of quantitative trait loci (QTL) interaction. Sex was taken as a binary trait, all bi-marker interactions were scanned with logistic regression model by using of high density microsatellite genetic linkage map. Results showed that 5 bi-marker combinations were detected at significance level of P＜0.05, among them, SCAFFOLD149_7459 and SCAFFOLD7854_71905 was significantly at P＜0.01 level, 5 bi-marker combinations came from 9 markers among 7 autosomes. These detected markers were mainly located on chromosome 2, 10 and 11, especially 2 bi-marker combinations located on linkage groups 10-11 and both possessed of SCAFFOLD149_7459, which indicating that this marker might have a greater impact on the process of interaction. This study showed that genes close to these markers were involved into sex determination of half-smooth tongue-sole, which means that autosomes may participate in the process of sex determination. The results have important meaning for further study sex determination and sex control of half-smooth tongue-sole.
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