Abstract：Wheat(Triticum aestivum)-Thinopyrum intermedium introgression line CH5383 is high resistance to wheat stripe rust and powdery mildew. It was characterized by genomic in situ hybridization (GISH), fluorescence in situ hybridization (FISH) and molecular marker analysis. GISH using Th.intermedium DNA as probe couldn't detect the alien signals. Strong hybridization signal in the distal region of wheat chromosome 3BL was observed when FISH using pHvG38 containing the simple GAA repeat as a probe. A total of 135 PLUG primer pairs were used to test in wheat Chinese Spring (CS), CH5383 and wheat related species. The results showed that TNAC1383 displayed unique 1 300 bp bands of CH5383 and Th. intermedium. Experimment also verified that CH5383 was a small wheat-alien introgression line and the alien Th. intermedium DNA segments were possibly located in wheat bin 3BL-0.81~1.00 region. Due to its good diseases resistance, CH5383 can be useful in breeding programs.
