Obtaining and Genetic Stability Analysis of Chinese Cabbage (Brassica oleracea var. capitata)-Cabbage (Brassica compestris ssp. pekinensis) Translocation Lines with Fragment of Cabbage Chromosome 7＃
Abstract： Translocation line is an individual formed by one-way or reciprocal translocation between chromosomes of two different species. It is significantly important for the application of excellent exogenous gene to study the genetic stability of exogenous chromosome in translocation lines. In order to obtain Chinese cabbage (Brassica oleracea var. capitata)-cabbage (Brassica compestris ssp. pekinensis) translocation lines, Chinese cabbage-cabbage alien addition line 7＃(AC7) and its parents Chinese cabbage 85-1 and cabbage 11-1 were used as experimental population in present study. Using rays 45 Gy 60Co-γ radiating pollens of AC7, M1 plants were obtained by backcrossing irradiated pollens with Chinese cabbage 85-1, and then M2 plants were obtained by selfing M1. Using 31 specific InDel (Insertion-Deletion) markers (compared with Chinese cabbage) distributed in linkage group No.1 of cabbage, and combining with cytological observation, ten translocation lines were identified from selfing progenies of M2 plants. Two translocation lines AT7-1 and AT7-2 with different chromosome fragments of cabbage were selected and studied, for which isolated microspore culture, selfing and backcrossing were further performed. Using specific InDel markers, progeny plants from selfing, backcrossing and microspore culture of two translocation lines were identified. We also had carried on investigation of phenotypic traits in selfing progenies of AT7-1 and AT7-2. The results showed that genetic stability of two translocation lines AT7-1 and AT7-2 with different chromosome fragments of cabbage was different. The genetic stability of selfing progenies, backcross progenies and microspore culture plants in the same translocation line was also different. In AT7-1, the ratio of keeping exogenous fragment in selfing progenies, backcross progenies and microspore culture plants was 73%, 28% and 50%, respectively. In AT7-2, the ratio of keeping exogenous fragment in selfing progenies, backcross progenies and microspore culture plants was 84%, 62% and 25%, respectively. Head formation related traits in selfing progenies of AT7-1 were segregated, but without segregation in selfing progenies of AT7-2. Studying genetic stability of exogenous chromosomes in Chinese cabbage background could be useful in using new germplasm for Chinese cabbage breeding.
[1]陈升位, 陈佩度, 王秀娥.利用电离辐射处理整臂易位系成熟雌配子诱导外源染色体小片段易位[J].科学通报, 2008, 38(3):215-220[2]Efremova T T, Maystrenko O I, Laykova L I.Development of alien substitution lines of wheat with rye chromosome 5R[J].Cereal Research Communications, 1996, 24(1):33-39[3]顾爱侠, 王彦华, 申书兴, 等.附加甘蓝 号染色体的大白菜单体异附加系的获得与研究[J].园艺学报, 2009, 36(1):39-44[4]胡永霞, 王彦华, 申书兴, 等.利用小孢子培养创建大白菜—结球甘蓝易位系[J].园艺学报, 2014, 41(7):1361-1368[5]孔 芳, 蒋金金, 吴 磊, 等.利用原位杂交及 标记分析芸薹属、和基因组间的关系[J].作物学报, 2008, 34(7):1188-1192[6]廖永翠, 宋 明, 王 辉, 等.大白菜中硫代葡萄糖苷的鉴定及含量分析[J].园艺学报, 2011, 38(5):963-969[7]李桂萍, 陈佩度, 张守忠, 等.小麦－簇毛麦易位染色体对小麦农艺性状的影响[J].植物遗传资源学报, 2011, 12(5):744-749[8]李宗芸, 栗茂腾, 黄荣桂, 等.基因组原位杂交辨别芸薹属异源四倍体、、基因组研究[J].中国油料作物学报, 2002, 22(1):10-14[9]Luan Y, Wang X G, Liu W H, et al.Production and identification of wheat-Agropyron cristatum 6P translocation lines[J].Planta, 2010, 232(2):501-510[10]吕文欣, 王彦华, 申书兴, 等.大白菜-结球甘蓝号染色体二体异附加系的获得与鉴定[J].园艺学报, 2011, 38(7):1275-1282[11]Naito K, Kusaba M, Shikazono N, et al.Transmissible and nontransmissible mutations induced by irradiating arabidopsis thaliana pollen with-rays and carbon ions[J].Genetics, 2005, 169(2):881-889[12]申书兴, 侯喜林, 张成合, 等.利用小孢子培养创建大白菜初级三体的研究[J].园艺学报, 2006, 33(6):1209-1214[13]王林生, 陈佩度, 王秀娥.普通小麦-大赖草染色体相互易位系T7DS·5LrL/T5LrS·7DL的分子细胞遗传学研究[J].科学通报, 2010, 55(8):669-674[14]王献平, 初敬华, 张相岐.小麦异源易位系的高效诱导和分子细胞遗传学鉴定[J].遗传学报, 2003, 30(7):619-624[15]Whelen E D, Conner P R L, Thomas J B.Transmission of a wheat alien chromosome translocation with resistance to the wheat curl mite in common wheat,Triticum trestivum L[J].Canada Journal of Genetics Cytology, 1986, 28(2):294-297[16]郄丽娟, 申书兴, 轩淑欣, 等.大白菜和结球甘蓝基因组原位杂交及核型分析[J].园艺学报, 2007, 34(6):1459-1464[17]轩淑欣, 张成合, 申书兴, 等.二倍体和四倍体结球甘蓝减数分裂观察[J].河北农业大学学报, 2008, 31(2):21-26[18]Zhao W C, Qi L L, Gao X, et al.Development and characterization of two new Triticum aestivum–Dasypyrum villosum Robertsonian translocation lines T1DS·1V#3L and T1DL·1V#3S and their effect on grain quality[J].Euphytica, 2010, 175(3):343-350[19]张文俊, 景健康, 胡 含, 等.黑麦染色体在小麦背景中的传递[J].遗传学报, 1995, 22(7):211-216[20]周文春, 赵寅槐, 邹明烈, 等.小麦型恢复系易位染色体在配子中的传递[J].江苏农业学报, 1997, 13(3):143-145[21]朱东旭, 王彦华, 申书兴, 等.结球甘蓝相对于大白菜连锁群特异标记的建立及应用[J].园艺学报, 2014, 41(8):1699-1706
