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2025年4月10日 星期四
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猪DPPA5基因cDNA全序列的克隆与分析*
李勇
中国农业科学院
Porcine DPPA5 Complete cDNA Cloning and Bioinformatics Analysis*
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摘要 摘要:本研究利用先前基于基因芯片技术构建的母猪妊娠阶段相关基因的调控网络,并结合生物信息学分析,筛选出一些与母猪妊娠相关的有价值的新候选基因。选取其中一个位于网络顶端的EST序列(CK455976)进行研究。根据此EST序列设计引物,以母猪卵巢为材料提取总RNA,运用RT-PCR技术和RACE-PCT技术对其进行cDNA全序列克隆,得到此基因cDNA全长596bp,并提交GenBank数据库,得到GenBank收录号FJ436413。经分析发现其中5`UTR长67bp,3`UTR长175bp,编码区长354bp,编码117个氨基酸。通过同源性比较预测了基因DNA序列全长约1123bp。利用同源性比对分析还发现,该基因核苷酸序列与人、小鼠和猕猴的同源性分别为83%、70%、83%;编码的氨基酸序列同源性分别为74%、55%和74%。经生物信息学分析,预测出DPPA5的相对分子量约为13.60KD,等电点为5.24,在大约10~19、24~42、47~77、84~90、101~118位之间氨基酸存在疏水性区域,分析结果还表明DPPA5为膜外蛋白。
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李勇
关键词 关键词:猪DPPA5基因序列克隆生物信息学分析    
Abstract:Abstract:The regulatory networks of the related genes at sow pregnancy stage was constructed by other in our laboratory, and some new and valuable candidate genes associated with sow pregnancy screened by bioinformatics analysis. A expressed sequence tag(EST) on the top of the networks was studied in the research. Primers were designed according to the sequence of the EST, the total RNA was extracted from sow ovaries,cDNA complete sequence cloned using RT-PCR and RACE-PCT was 596bp, the sequence was submitted and the accession number(FJ36413) was got from the GenBank database. The complete sequence included untranslated region( located in 1-67 and 422-596) and coding region(located in 65-421) coding a polypeptide of 117 amino acids. The predicted nucleotide sequence similarity between pig and human, mouse, and macaca mulatta were83%, 70%, 83%, amino acid sequence were 74%, 55%, 74%, respectively. The relative molecular weight of predicted DPPA5 was 13.60KD , and its isoelectric point was 5.24. The hydrophobic region located in 10-19, 24-42, 47-77, 84-90, and 101-118. The analysis result indicated that DPPA5 was an extrinsic protein.
Key wordsKey words:pig    DPPA5    cloning the sequences    bioinformatics analyses
收稿日期: 2009-02-12     
通讯作者: 李勇   
引用本文:   
李勇. 猪DPPA5基因cDNA全序列的克隆与分析*[J]. , 2010, 18(2): 285-289.
链接本文:  
http://journal05.magtech.org.cn/Jwk_ny/CN/     或     http://journal05.magtech.org.cn/Jwk_ny/CN/Y2010/V18/I2/285
 
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