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EGF对牛前脂肪细胞增殖和分化的影响
郭红芳1,昝林森2
1.
2. 西北农林科技大学动物科技学院
Effect of EGF on proliferation and differentiation of bovine preadipocytes
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摘要 摘要:本研究以分离新生牛腹股沟白色脂肪组织的前体脂肪细胞进行原代培养为基础,采用MTT比色法和油红O提取比色法研究10 ng/ml、50 ng/ml、100 ng/ml、200 ng/ml 表皮生长因子(EGF)对牛前脂肪细胞增殖及分化的影响,同时通过半定量RT-PCR检测不同浓度EGF处理细胞第8天时对分化标志基因脂蛋白酯酶(Lipoprotein lipase, LPL)、过氧化物酶体增殖物激活受体γ(Peroxisome proliferator-activated receptorγ,PPARγ)、脂肪细胞脂肪酸结合蛋白(adipocyte fatty-acid-binding protein,A-FABP)mRNA 表达的影响。结果表明,10ng/ml EGF能极显著促进前脂肪细胞的增殖(P<0.01),在分化诱导后添加不同浓度的EGF在第8天均可促进脂肪细胞的分化,并能促进脂肪细胞分化标志基因LPL、PPARγ和A-FABP的表达。因此,EGF可以促进牛前脂肪细胞的增殖和分化。
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郭红芳
昝林森
关键词 关键词: 牛前脂肪细胞EGF增殖和分化标志基因    
Abstract:abstract: The study was to investigate the effect of EGF on Bovine preadipocytes which were isolated from bovine perirenal adipose. Effect of doses 10 ng/ml、50 ng/ml、100 ng/ml、200 ng/ml EGF on proliferation and differentiation of preadipocytes were analyzed by MTT colorimetric and incorporation of oil red O. and mRNA expression levels of LPL,PPARγ,A-FABP genes,which were adipocyte differentiated marker genes were determined by semi-quantitative RT-PCR. The preadipocyte proliferation was stimulated significantly by EGF of 10ng/ml (P<0.01). Treated with different doses EGF after induced differentiation could improve adipocyte differentiation and promote the expression of the differentiation marker genes LPL 、PPARγand A-FABP at 8th day .EGF could improve the proliferation and differentiation of bovine preadipocyte.
Key wordsKey words:Bovine    preadipocyte    EGF    proliferation and differentiation    marker gene
收稿日期: 2009-01-13     
通讯作者: 昝林森   
引用本文:   
郭红芳1,昝林森2. EGF对牛前脂肪细胞增殖和分化的影响[J]. , 2010, 18(2): 290-295.
链接本文:  
http://journal05.magtech.org.cn/Jwk_ny/CN/     或     http://journal05.magtech.org.cn/Jwk_ny/CN/Y2010/V18/I2/290
 
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