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2025年4月3日 星期四
  研究论文与报告 本期目录 | 过刊浏览 | 高级检索 |
天祝白牦牛金属硫蛋白基因(MT-1)全序列分子克隆及生物信息学分析
王雪莹1,姬菩忠2,马琦1
1. 甘肃农业大学
2. 兰州军区总医院
Cloning and Bioinformatic Analysis of Metallothionein-1 Gene (MT-1) in Tianzhu White Yak(Bos grunneins)
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摘要 金属硫蛋白-1基因(metallothionein-1, MT-1)是一类广泛存在于动物体内的金属结合蛋白,除维持金属动态平衡和重金属解毒外,还可参与清除自由基、拮抗电离辐射和抗应激等。为深入研究MT-1基因参与动物机体抗逆性的分子机制,本研究采用RT-PCR和RACE (rapid amplification of cDNA ends,RACE)技术获得天祝白牦牛(Bos grunneins)MT-1基因全长cDNA序列,运用生物信息学方法分析MT-1蛋白的理化性质、结构和不同物种间的同源性,并利用RT-qPCR方法分析MT-1基因在天祝白牦牛各个脏器和组织中的表达丰度。结果表明,天祝白牦牛MT-1基因cDNA序列全长为408 bp(GenBank登录号: KF770836),开放阅读框(ORF)长186 bp,编码61个氨基酸,氨基酸的分子量为5.981 kD;氨基酸序列分析显示,天祝白牦牛MT-1基因编码氨基酸序列与牛(Bos taurus)的同源性高达100%,同时与大多数哺乳动物相似;荧光定量PCR结果发现,MT-1 mRNA在天祝白牦牛8个不同脏器和组织中均有表达,心脏中表达量最高。天祝白牦牛MT-1基因的成功克隆为进一步研究该基因的功能提供了基础资料。
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王雪莹
姬菩忠
马琦
关键词 金属硫蛋白1天祝白牦牛RACE生物信息学分析    
Abstract:Metallothionein-1(MT-1) is an ubiquitously dispersed metal-binding protein in animals, which serves in maintaining metal homeostasis and heavy metal detoxification, and also scavenges free radicals, resists ionize radiation and anti-stress. For further research on the molecular mechanism of MT-1 gene involved in stress resistance, full length cDNA sequence of MT-1 gene in Tianzhu white yak(Bos grunneins) was cloned by RT-PCR and RACE (rapid amplification of cDNA ends). Physicochemical property, structure and homology of MT-1 encoded protein were analyzed by bioinformatic methods. Real-time PCR was used to analyze MT-1 mRNA expression profile in different tissues of Tianzhu white yak. The result showed that the full length of MT-1 cDNA was 408 bp (GenBank accession No. KF770836), with an ORF (open reading frame) of 182 bp in length, which encoded 61 amino acids with a predicted molecular mass of 5.981 kD. The predicted amino acid sequences showed 100% similarity with Bos taurus, and was highly homologous with most mammalians. The mRNA expression of MT-1 gene was found in 8 different tissues of Tianzhu white yak by Real-time PCR, the highest expression level was found in heart. Therefore, the full length cDNA of MT-1 gene was successfully obtained, which provides basic data for further study on its function.
Key wordsMetallothionein-1(MT-1) gene    Tianzhu white yak    Rapid-amplification of cDNA ends (RACE)    Bioinformatic analysis
收稿日期: 2014-01-17     
通讯作者: 马琦   
引用本文:   
王雪莹1,姬菩忠2,马琦1. 天祝白牦牛金属硫蛋白基因(MT-1)全序列分子克隆及生物信息学分析[J]. , 2014, 22(7): 883-891.
链接本文:  
http://journal05.magtech.org.cn/Jwk_ny/CN/     或     http://journal05.magtech.org.cn/Jwk_ny/CN/Y2014/V22/I7/883
 
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