Expression Analysis of a Stress Repressed Gene OsDSR4 from DUF966 Family and Generation of OsDSR4-overexpressing Transgenic Rice (Oryza sativa L. ssp. japonica)
Abstract：OsDSR4 is a gene of unkown function in DUF966 gene family, and the function of DUF966 family genes have not been reported until now. In this study, the bioinformatic analysis showed that the cDNA of OsDSR4 had 2 167 bp containing an open reading frame (ORF) of 1 149 bp, and it encoded a putative protein of 372 amino acids with a highly conserved DUF966 domain. The gene expression profile analysis indicated that OsDSR4 was expressed mainly in internode and leaf blade of rice(Oryza sativa L.), and it was repressed markedly by drought, salt and cold stresses, and induced significantly by abscisic acid(ABA). OsDSR4 was cloned using overlap extension PCR, and the fusion construct containing OsDSR4 was introduced into rice(Oryza sativa L. ssp. japonica) by Agrobacterium-mediated transformation method. Thirty-two OsDSR4-overexpressing transgenic plants were obtained and identified by PCR and qRT-PCR, which was demonstated that OsDSR4 had been integrated into rice genome and was overexpressed in some positive transgenic plants. These results establish the foundation for further study of the precise function of OsDSR4.
