Cloning, Expression, and Function Analysis Under Ralstonia solanacearum Stress of Pathogenesis-related Protein SlPR1b Gene in Tomato (Solanum lycopersicum)
CHEN Na1, LI Xiao-Peng1, LIU Jin-Fa2, SHAO Qin1,*
1 College of Life Science and Resources and Environment, Yichun University, Yichun 336000, China; 2 Management Committee of Modern Agricultural Science Park of Xinyu, Xinyu 338000, China
Abstract：Pathogenesis-related protein 1 (PR1) gene plays an important role in plant resistance to biotic and abiotic stresses. Based on the transcriptome data of Ralstonia solanacearum stress previously conducted by our research group, in this study, a PR1 gene, named SlPR1b (Solyc00g174340.2) was cloned from tomato (Solanum lycopersicum) by reverse transcription PCR (RT PCR) technology. qPCR was used to reveal SlPR1b gene tissue expression specificity and expression characteristic under the conditions including infection with Ralstonia solanacearum, and treatment with salicylic acid (SA) and methyl jasmonate acid (MeJA). SlPR1b gene virus induced gene silencing (VIGS) vector was constructed and then transformed into tomato resistance material to analyze the resistance ability of SlPR1b gene silenced tomato in R. solanacearum stress condition. The results showed that the full-length cDNA sequence of SlPR1b gene was 807 bp, its ORF was 480 bp, encoding 159 amino acids, including a conserved CAP-PR-1 domain (cd05381), belonging to the CAP superfamily. The predicted molecular weight of SlPR1b was 17 519.73 D, the isoelectric point was 8.86, and the protein was found to be a secreted protein with a transmembrane structure. Homologous sequence alignment and phylogenetic analysis indicated that SlPR1b was highly homologous with a S. pennellii SpPR4 protein, followed by S. tuberosum StPR1b protein. The results of tissue specific expression showed that SlPR1b gene expression level was the highest in tomato leaves. Furthermore, SlPR1b gene expression could be induced by R. solanacearum, SA, and MeJA. Silencing SlPR1b decreased plant resistance to bacterial wilt, these results suggested that SlPR1b played a positive role in tomato resistance to bacterial wilt. This study provided a reference for further exploring the role of SlPR1b gene in the response of tomato to bacterial wilt.
陈娜, 李晓鹏, 刘进法, 邵勤. 番茄病程相关蛋白SlPR1b基因的克隆、表达及其在青枯菌胁迫下的功能分析[J]. 农业生物技术学报, 2023, 31(12): 2477-2489.
CHEN Na, LI Xiao-Peng, LIU Jin-Fa, SHAO Qin. Cloning, Expression, and Function Analysis Under Ralstonia solanacearum Stress of Pathogenesis-related Protein SlPR1b Gene in Tomato (Solanum lycopersicum). 农业生物技术学报, 2023, 31(12): 2477-2489.
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