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2025年8月21日 星期四
农业生物技术学报  2023, Vol. 31 Issue (6): 1133-1146    DOI: 10.3969/j.issn.1674-7968.2023.06.003
  研究论文与报告 本期目录 | 过刊浏览 | 高级检索 |
ZlPIN1a 基因克隆及其在菰黑粉菌侵染诱导菰茎部发育中的表达分析
虞悦*, 周美琪*, 戚晓清, 柳菁溪, 张雅芬, 叶子弘, 崔海峰**
中国计量大学 生命科学学院/浙江省生物计量与检验检疫技术重点实验室,杭州 310018
Cloning of the ZlPIN1a Gene and its Expression Analysis in Stem Development of Zizania latifolia Induced by Infection of Ustilago esculenta
YU Yue*, ZHOU Mei-Qi* , QI Xiao-Qing , LIU Jin-Xi , ZHANG Ya-Fen , YE Zi-Hong , CUI Hai-Feng**
Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine/College of Life Sciences, China Jiliang University, Hangzhou 310018, China
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摘要 菰黑粉菌(Ustilago esculenta)侵染菰(Zizania latifolia)植株后可诱导植株茎部膨大发育,顶端生长 受到显著抑制,这可能与茎部3-吲哚乙酸(3-indoleacetic acid, IAA)极性运输调控相关。本研究克隆获得了6个茭白'浙茭7号' IAA 极性运输输出载体相关基因 ZlPINs (Z. latifolia PIN formed),这6个基因均具有 PIN (PIN formed)蛋白家族的 Mem_trans 结构域, 其中 ZlPIN1a、ZlPIN1b、ZlPIN2 及 ZlPIN3a 均与野生稻 (Oryza brachyantha)氨基酸的同源性较高,相似度分别高达 96.80%、89.27%、90.52%及82.88%。菰植株组 织特异性表达分析发现, 6个 ZlPINs 基因均可在根部高表达(P<0.05);在叶片和叶鞘中表达量较低 ; ZlPIN1aZlPIN1bZlPIN4 可在茎部表达, 其中ZlPIN1a表达量显著高于其他 ZlPINs 基因(P<0.05), 推测 ZlPIN1a 可能是菰植株茎部 IAA 极性运输的主要调控基因。ZlPIN1a 基因(GenBank No. OM782294)克 隆序列全长为 1 770 bp,编码 589 个氨基酸,有6个外显子和5个内含子; 菰黑粉菌体外侵染表明,菰植株茎部 ZlPIN1a 基因的表达量受菰黑粉菌侵染的抑制调节(P<0.05),正常茭和灰茭植株的膨大发育初期, 茎部表达量均显著低于雄茭植株(P<0.05);茭白肉质茎部膨大发育进程中, 正常茭茎部 ZlPIN1a 的表达量先升高后降低,而灰茭茎部的表达量持续升高,ZlPIN1a 表达变化可能与菰黑粉菌侵染诱导的2种茎部膨大发育表型相关。本研究筛选获得了菰植株茎部表达的 IAA 极性运输相关基因 ZlPIN1a,明确了菰黑粉菌侵染对其在茎部表达的抑制调节,探讨了其在肉质茎膨大发育中的表达模式,为菰黑粉菌侵染诱导 菰植株茎部膨大发育的调节机制研究提供新的思路。
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虞悦
周美琪
戚晓清
柳菁溪
张雅芬
叶子弘
崔海峰
关键词 菰黑粉菌茎部膨大发育3-吲哚乙酸(IAA)极性运输表达模式    
Abstract:Zizania latifolia stem swelling is caused by infection of Ustilago esculenta, following the inhibition of apical dominance of the stem, which may be related to the regulation of polar transportation of auxin. In this research, 6 ZlPINs (Z. latifolia PIN formed) of indole-3-aceticacid (IAA) polar transport vector related genes were cloned from Jiaobai 'Zhejiao 7'. The Mem_trans structural domains were found among the 6 ZlPINs. Phylogenetic tree analysis showed that ZlPIN1a, ZlPIN1b, ZlPIN2 and ZlPIN3a were highly similar to wild rice (Oryza brachyantha) with the similarity of 96.80%, 89.27%, 90.52% and 82.88% respectively. The expression of different tissues of Z.latifolia showed that all of the 6 ZlPINs were significantly higher expressed in root than those in leaf and sheath (P<0.05). The expression of ZlPIN1a, ZlPIN1b and ZlPIN4 could be detected in the stem, while the expression level of ZlPIN1a was significantly higher than other ZlPINs genes (P<0.05) . It was speculated that ZlPIN1a would be the major regulatory gene for the polar transport of IAA in the stem of Z. latifolia. The cloned sequence of ZlPIN1a (GenBank No. om782294) had a total length of 1 770 bp and encoded 589 amino acids, including 6 exons and 5 introns. Based on the infection of U. esculenta in Z. latifolia in vitro, it was showed that the expression of ZlPIN1a in stem was inhibited by the infection of U. esculenta (P<0.05). Compared to Male Jiaobai, the significantly higher expression of ZlPIN1a in stem were found in Normal Jiaobai and Grey Jiaobai (P<0.05). During the development of swollen stem, the expression of ZlPIN1a in Normal Jiaobai increased firstly and then decreased, however, the expression of ZlPIN1a increased continuously in Grey Jiaobai. The expression change of ZlPIN1a would be related to the 2 stem swollen development phenotypes induced by the infection of U. esculenta. In this research, ZlPIN1a was screened as the IAA polar transport related gene expressed in the stem of Z. latifolia, which could be inhibited by the infection of U. esculenta, The expression mode of ZlPIN1a in the development of swollen stem was discussed, which could provide a new idea for the regulation mechanism of swollen stem caused by the infection of U. esculenta in Z. latifolia.
Key wordsZizania latifolia    Ustilago esculenta    Swelling development of stem    3-indoleacetic acid (IAA) polarity transport    Expression pattern
收稿日期: 2022-03-15     
通讯作者: **hfcui@cjlu.edu.cn   
引用本文:   
虞悦, 周美琪, 戚晓清, 柳菁溪, 张雅芬, 叶子弘, 崔海峰. ZlPIN1a 基因克隆及其在菰黑粉菌侵染诱导菰茎部发育中的表达分析[J]. 农业生物技术学报, 2023, 31(6): 1133-1146.
YU Yue, ZHOU Mei-Qi,QI Xiao-Qing,LIU Jin-Xi,ZHANG Ya-Fen,YE Zi-Hong,CUI Hai-Feng. Cloning of the ZlPIN1a Gene and its Expression Analysis in Stem Development of Zizania latifolia Induced by Infection of Ustilago esculenta. 农业生物技术学报, 2023, 31(6): 1133-1146.
链接本文:  
http://journal05.magtech.org.cn/Jwk_ny/CN/10.3969/j.issn.1674-7968.2023.06.003     或     http://journal05.magtech.org.cn/Jwk_ny/CN/Y2023/V31/I6/1133
 
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