Expression Characteristics and Correlation Analysis of HDAC6 and AREG of Different Developmental Stages Follicles in Tan Sheep Ovarian (Ovis aries)
FAN Shan-Shan1,2, LIU Yu-Jun1,2, XU Ya-Xiu1,2, YANG Yi1,2, LIU Xin-Feng1,2,*, WANG Chao3,*
1 Key laboratory of Education for Conservation and Utilization of Special Biological Resources in the Western, Ningxia University, Yinchuan 750021, China; 2 College of Life Sciences, Ningxia University, Yinchuan 750021, China; 3 State Key Laboratory of Agricultural Biotechnology, College of Biology, China Agricultural University, Beijing 100193, China
Abstract：Histone deacetylase 6 (HDAC6) is an important epigenetic modification protein and amphiregulin (AREG) is an important epithelial growth factor-like (EGF-like). HDAC6 and AREG play important roles in regulating mammalian reproduction. This study aims to explore the expression rules of HDAC6 and AREG in ovarian follicles with different development stages of Tan sheep (Ovis aries) and analyze their correlation in follicle development, in this study, the ovaries of Tan sheep were collected for detecting the localization and expression patterns of HDAC6 and AREG in ovarian follicles with different development stages of Tan sheep by immunohistochemistry, immunofluorescence and Western blot methods, and the correlation between HDAC6 and AREG in follicular development was analyzed by Pearson method. The results showed that HDAC6 was expressed in both granulosa cells and oocytes of ovarian follicles at different developmental stages of Tan sheep, and was mainly expressed in the cytoplasm of granulosa cells and oocytes. With the development of follicles, the expression level of HDAC6 increased first and then decreased in follicles with different development stages, and the expression level of HDAC6 in follicles larger than 6 mm in diameter was significantly lower than that in follicles less than 2 mm in diameter. AREG was mainly expressed in granulosa cells of different sizes of ovarian follicles of Tan sheep, and was mainly expressed in granulosa cytoplasm. With the development of follicles, the expression level of AREG was gradually increased in follicles with different development stages, and the expression level of AREG in follicles with diameters greater than 6 mm was significantly higher than that in follicles with diameters less than 2 mm. Furthermore, the correlation analysis found that the expression level of HDAC6 was negatively correlated with that of AREG in follicles with different diameters of Tan sheep. The expression level of HDAC6 in follicles with diameters of less than 2 mm was moderately negatively correlated with that of AREG, and the expression level of HDAC6 in follicles with diameters of 2~6 mm was strongly negatively correlated. The expression level of AREG was negatively correlated with HDAC6 in follicles larger than 6 mm with diameter. The results showed that HDAC6 and AREG were expressed in ovarian follicles with different development stages of Tan sheep, and the expression patterns of HDAC6 and AREG were negatively correlated in general, suggesting that HDAC6 and AREG may be involved in ovarian follicle development of Tan sheep through negative correlation regulation. This study provides an important reference for further exploring the expression relationship of HDAC6 and AREG in granulosa cells and oocytes at different development stages of Tan sheep ovarian follicles and their effects on follicular development and oocyte maturation, and possible mechanisms.
范闪闪, 柳雨均, 徐娅秀, 杨易, 刘新峰, 王超. HDAC6与AREG在滩羊卵巢不同发育阶段卵泡中的表达特征及相关性分析[J]. 农业生物技术学报, 2022, 30(8): 1524-1533.
FAN Shan-Shan, LIU Yu-Jun, XU Ya-Xiu, YANG Yi, LIU Xin-Feng, WANG Chao. Expression Characteristics and Correlation Analysis of HDAC6 and AREG of Different Developmental Stages Follicles in Tan Sheep Ovarian (Ovis aries). 农业生物技术学报, 2022, 30(8): 1524-1533.
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