Autophagy Participates in Abscisic Acid-mediated Salt Tolerance in Wheat (Triticum aestivum)
LI Yong-Bo1,*, CUI De-Zhou1,*, HUANG Chen1, SUI Xin-Xia1, FAN Qing-Qi1,**, CHU Xiu-Sheng1,2,**
1 Crop Research Institute, Shandong Academy of Agricultural Sciences/Key Laboratory of Wheat Biology and Genetic Improvement in North Yellow and Huai River Valley, Ministry of Agriculture/National Engineering Laboratory for Wheat and Maize, Jinan 250100, China; 2 School of Life Science, Shandong Normal University, Jinan 250014, China
Abstract：Autophagy is a self-defense mechanism under environmental stress in plants. It has been proved that autophagy is involved in salt tolerance of wheat (Triticum aestivum), but the role and regulation mechanism of autophagy in salt tolerance are not clear. In this study, salt-tolerant wheat 'DEKANG961' and salt-intolerant wheat 'LANKAO323' were used as materials, and the methods of lysosomes' fluorescence probe, Western blot and so on were used to detect autophagy level. It was found that salt stress could promote autophagy, but the autophagy level of 'DEKANG961' was higher than that of 'LANKAO323', the gene expression of PYL1 (pyrabactin), PYL2, PYL3 and sucrose non-fermenting related protein kinase 2 (SNRK2), the key genes of abscisic acid (ABA) signaling pathway in 'DEKANG961', were significantly higher than that in 'LANKAO323', and the autophagy level of 'DEKANG961' was significantly higher than that of 'LANKAO323'. It was concluded that ABA signal pathway could promote the autophagy to enhance salt tolerance in wheat. Therefore, this study has certain theoretical and practical significance for further study on salt-tolerant mechanism of wheat and breeding new salt-tolerant wheat varieties.
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