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2025年8月6日 星期三
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水杨酸诱导玉米对大斑病抗性的研究
张莹 王艳辉 郝敏 韩建民 董金皋 贾慧
河北农业大学生命科学学院 河北农业大学生命科学学院 河北北方学院医学技术学院 河北农业大学生命科学学院 河北农业大学生命科学学院 河北农业大学生命科学学院
Study on salicylic acid induced resistance to Exserohilum turcicum
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摘要 外施SA可以显著减轻玉米大斑病的病害症状。表现为玉米叶片的病斑数目和病斑大小明显降低,诱抗效果超过56%。SA诱导玉米抗病机制研究表明:SA预处理,减缓了玉米大斑病菌侵染速度,表现为由侵入钉长出新菌丝,菌丝充满侵染细胞以及向邻近细胞扩展的时间推迟。SA预处理加速了病菌侵染过程中侵染点及周围玉米细胞的死亡。SA处理或SA预处理后接菌,明显增强了玉米叶片中PAL、几丁质酶、β-1,3-葡聚糖酶活性;增加了玉米叶片内木质素、丁布的含量;而单独接菌,这些酶活性及物质含量均无明显改变。SA处理后接菌,增加了玉米叶片内H2O2含量。
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张莹王艳辉郝敏韩建民董金皋贾慧
关键词 水杨酸 玉米 玉米大斑病菌 玉米大斑病 诱导抗病性    
Abstract:Northern corn leaf blight caused by Exserohilum turcicum was inhibited by spraying leaves with salicylic acid (SA) solution in greenhouse test. The tests indicated that: the lesion amount and lesion area on the leaf could be reduced in the maize seedlings (OH43, OH43Ht1, Huangzao4, Huangzao4Ht1) sprayed continuously for 6 days with 10mM SA, which had the effect of inhibition above56% generally. The study on physiological mechanism of resistance to Northern Corn Leaf Blight showed the infection speed of E. turcicum was slowed in the maize leaves pretreated by 10mM SA. Haustorium formation from papilla and the growth of haustoriun in the leaf cells were delayed compared with the water-control. The pretreatment of SA also induced and promoted cell death in the infected cell as well as the adjacent. The activities of phenylalanine amonnialyase (PAL), chitinase and β-1,3-glucanase of maize leaves were enhanced significantly with SA treatment or treatment of E. turcicum inoculation after SA pretreatment, the content of lignin and DAMBOA in the maize leaves were also increased. Increasing of H2O2 levels was also observed in the maize leaves with the treatment of E. turcicum inoculation after SA inducing.
Key wordssalicylic acid    maize    Exserohilum turcicum    Northern Corn Leaf Blight    induced resistance
收稿日期: 2007-06-14     
通讯作者: 韩建民   
引用本文:   
张莹 王艳辉 郝敏 韩建民 董金皋 贾慧. 水杨酸诱导玉米对大斑病抗性的研究[J]. , 2008, 16(3): 0-.
. Study on salicylic acid induced resistance to Exserohilum turcicum. , 2008, 16(3): 0-.
链接本文:  
http://journal05.magtech.org.cn/Jwk_ny/CN/      或     http://journal05.magtech.org.cn/Jwk_ny/CN/Y2008/V16/I3/0
 
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