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2025年8月3日 星期日
农业生物技术学报  2021, Vol. 29 Issue (4): 764-771    DOI: 10.3969/j.issn.1674-7968.2021.04.014
  研究论文与报告 本期目录 | 过刊浏览 | 高级检索 |
玉米大斑病菌交配型基因StMAT1-1StMAT1-2表达与转录活性分析
杨贝贝1, 杨阳2, 戴冬青1, 刘宁3,4, 贾慧4, 曹志艳3,4,*, 董金皋3,4,*
1 河北农业大学 生命科学学院, 保定 071000;
2 河北省农林科学院 经济作物研究所, 石家庄 050051;
3 河北农业大学 植物保护学院, 保定 071000;
4 华北作物改良与调控国家重点实验室, 保定 071000
Expression and Transcriptional Activity Analysis of Mating Type Genes StMAT1-1 and StMAT1-2 in Setosphaeria turcica
YANG Bei-Bei1, YANG Yang2, DAI Dong-Qing1, LIU Ning3,4, JIA Hui4, CAO Zhi-Yan3,4,*, DONG Jin-Gao3,4,*
1 College of Life Science, Hebei Agricultural University, Baoding 071000, China;
2 Institute of Cash Crops, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050051, China;
3 College of Plant Protection, Hebei Agricultural University, Baoding 071000, China;
4 State Key Laboratory of North China Crop Improvement and Regulation, Baoding 071000, China
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摘要 玉米大斑病菌(Setosphaeria turcica)的遗传变异源于病菌的有性生殖,为进一步探究玉米大斑病菌交配型基因在有性生殖中的作用,本研究对带有不同交配型(mating type, MAT)基因 StMAT1-1StMAT1-2的玉米大斑病菌菌株进行有性态诱导,采用qRT-PCR分析不同交配型菌株在有性态诱导0、15、30和60 d时StMAT1-1StMAT1-2基因的表达规律,同时通过酵母(Saccharomyces cerevisiae)自激活试验检测不同交配型基因的转录激活活性。结果表明,有性态诱导前,有性态诱导15、30和60 d时StMAT1-1StMAT1-2基因均有表达,且随着诱导时间延长,交配型基因在不同诱导组合中的表达量也随着时间的增加而显著变化,说明在玉米大斑病菌有性生殖过程中交配型基因起着重要的作用;酵母自激活实验发现,StMAT1-1StMAT1-2基因编码产物均为转录因子,且具有较高的转录激活活性。研究结果为进一步探究交配型基因在调控玉米大斑病菌有性生殖过程中发挥的关键作用提供参考。
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杨贝贝
杨阳
戴冬青
刘宁
贾慧
曹志艳
董金皋
关键词 玉米大斑病菌有性态诱导交配型基因自激活    
Abstract:The genetic variation of Setosphaeria turcica was mainly caused by sexual reproduction. In order to explore the role of the S. turcica mating type (MAT) genes StMAT1-1 and StMAT1-2 in sexual reproduction, some strains with different mating type genes were sexually induced, and qRT-PCR was used to analyze the expression patterns of StMAT1-1 and StMAT1-2 in different mating type strains at 0, 15, 30 and 60 d after sexual induction. The transcriptional activity of StMAT1-1 and StMAT1-2 was determined by yeast (Saccharomyces cerevisiae) self-activation test. The results showed that StMAT1-1 and StMAT1-2 genes were expressed before sexual induction, 15, 30 and 60 d after sexual induction. With the extension of induction time, the expression of mating type genes in different induction combinations also changes significantly, indicating that MAT genes played an important role in the sexual reproduction process of S. turcica. In yeast self-activation test, it was found that the encoded proteins of StMAT1-1 and StMAT1-2 genes were transcription factors with high transcriptional activation activity. This study has reference value to the further research on the key role of MAT genes in the regulation of sexual reproduction of S. turcica.
Key wordsSetosphaeria turcica    Sexual induction    Mating type gene    Self-activation
收稿日期: 2020-06-28      出版日期: 2021-04-01
ZTFLH:  S435.131  
基金资助:现代农业产业技术体系专项(CARS-02)
通讯作者: * caozhiyan@hebau.edu.cn;shmdjg@hebau.edu.cn   
引用本文:   
杨贝贝, 杨阳, 戴冬青, 刘宁, 贾慧, 曹志艳, 董金皋. 玉米大斑病菌交配型基因StMAT1-1StMAT1-2表达与转录活性分析[J]. 农业生物技术学报, 2021, 29(4): 764-771.
YANG Bei-Bei, YANG Yang, DAI Dong-Qing, LIU Ning, JIA Hui, CAO Zhi-Yan, DONG Jin-Gao. Expression and Transcriptional Activity Analysis of Mating Type Genes StMAT1-1 and StMAT1-2 in Setosphaeria turcica. 农业生物技术学报, 2021, 29(4): 764-771.
链接本文:  
http://journal05.magtech.org.cn/Jwk_ny/CN/10.3969/j.issn.1674-7968.2021.04.014     或     http://journal05.magtech.org.cn/Jwk_ny/CN/Y2021/V29/I4/764
 
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