Expression and Transcriptional Activity Analysis of Mating Type Genes StMAT1-1 and StMAT1-2 in Setosphaeria turcica
YANG Bei-Bei1, YANG Yang2, DAI Dong-Qing1, LIU Ning3,4, JIA Hui4, CAO Zhi-Yan3,4,*, DONG Jin-Gao3,4,*
1 College of Life Science, Hebei Agricultural University, Baoding 071000, China; 2 Institute of Cash Crops, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050051, China; 3 College of Plant Protection, Hebei Agricultural University, Baoding 071000, China; 4 State Key Laboratory of North China Crop Improvement and Regulation, Baoding 071000, China
Abstract:The genetic variation of Setosphaeria turcica was mainly caused by sexual reproduction. In order to explore the role of the S. turcica mating type (MAT) genes StMAT1-1 and StMAT1-2 in sexual reproduction, some strains with different mating type genes were sexually induced, and qRT-PCR was used to analyze the expression patterns of StMAT1-1 and StMAT1-2 in different mating type strains at 0, 15, 30 and 60 d after sexual induction. The transcriptional activity of StMAT1-1 and StMAT1-2 was determined by yeast (Saccharomyces cerevisiae) self-activation test. The results showed that StMAT1-1 and StMAT1-2 genes were expressed before sexual induction, 15, 30 and 60 d after sexual induction. With the extension of induction time, the expression of mating type genes in different induction combinations also changes significantly, indicating that MAT genes played an important role in the sexual reproduction process of S. turcica. In yeast self-activation test, it was found that the encoded proteins of StMAT1-1 and StMAT1-2 genes were transcription factors with high transcriptional activation activity. This study has reference value to the further research on the key role of MAT genes in the regulation of sexual reproduction of S. turcica.
杨贝贝, 杨阳, 戴冬青, 刘宁, 贾慧, 曹志艳, 董金皋. 玉米大斑病菌交配型基因StMAT1-1和StMAT1-2表达与转录活性分析[J]. 农业生物技术学报, 2021, 29(4): 764-771.
YANG Bei-Bei, YANG Yang, DAI Dong-Qing, LIU Ning, JIA Hui, CAO Zhi-Yan, DONG Jin-Gao. Expression and Transcriptional Activity Analysis of Mating Type Genes StMAT1-1 and StMAT1-2 in Setosphaeria turcica. 农业生物技术学报, 2021, 29(4): 764-771.
[1] 戴冬青, 王绍新, 刘宁, 等. 2019. 玉米大斑病菌交配型组成鉴定及其有性生殖条件优化[J].植物保护学报,46(03): 634-641. (Dai D Q, Wang S X, Liu N, et al.2019. Identification of mating type composition and optimization of sexual reproduction conditions of Setosphaeria turcica[J]. Journal of Plant Protection, 46(03): 634-641) [2] 刘杰, 姜玉英, 曾娟. 2013. 2012年玉米大斑病重发原因和控制对策[J]. 植物保护, 39(6): 86-90. (Liu J, Jiang Y Y, Zeng J.2013. Causes and control strategies of corn leaf spot in 2012[J]. Plant Protection, 39(6): 86-90) [3] 刘克心. 2018. 铁离子对新月弯孢菌(Curvularia lunata)有性世代发育的影响[D]. 硕士学位论文, 沈阳农业大学, 导师: 薛春生, pp. 54-58. (Liu K X.2018. Effects of iron ions on the sexual generation of Curvularia lunata[D]. Thesis for M.S., Shenyang Agriculture University, Supervisor: Xue C S, pp. 54-58.) [4] 王慧慧, 张文忠, 芦明, 等. 2016. 玉米大斑病的研究进展[J]. 天津农业科学, 22(12): 133-136. (Wang H H, Zhang W Z, Lu M, et al.2016. Advances in research on corn leaf spot[J]. Tianjin Agricultural Sciences, 22(12): 133-136) [5] 杨阳, 马双新, 贾慧, 等. 2015. 玉米大斑病菌两性交配型菌株的出现频率及其育性分析[J]. 植物保护学报, 42(6): 921-926. (Yang Y, Ma S X, Jia H, et al.2015. The occurrence and fertility analysis of bisexual strains of Setosphaeria turcica[J]. Journal of Plant Protection, 42(6): 921-926) [6] Böhm J, Hoff B, O'Gorman CM, et al.2013. Sexual reproduction and mating-type-mediated strain development in the penicillin-producing fungus Penicillium citrinum[J]. Proceedings of the National Academy of Sciences of the USA, 110(4): 1476-1481. [7] Chitrampalam P, Inderbitzin P, Maruthachalam K, et al.2013. The Sclerotinia sclerotiorum mating type locus (MAT) contains a 3.6-kb region that is inverted in every meiotic generation[J]. PLOS ONE, 8(2): e56895. [8] Coppin E, de Renty C, Debuchy R, 2005. The function of the coding sequences for the putative pheromone precursors in Podospora anserine is restricted to fertilization[J]. Eukaryot Cell, 4(2): 407-420. [9] Czaja W, Miller K Y, Skinner M K, et al.2014. Structural and functional conservation of fungal Mat A and human SRY sex-determining proteins[J]. Nature Communications, 5: 5434. [10] De Miccolis Angelini R M, Rotolo C, Pollastro S, et al.2016. Molecular analysis of the mating type (MAT1) locus in strains of the heterothallic ascomycete Botrytis cinerea[J]. Plant Pathology, 65(8): 1321-1332. [11] Fraser J A, Heitman J.2005. Chromosomal sex-determining regions in animals, plants and fungi[J]. Current Opinion in Genetics and Devolopment, 15(6): 645-651. [12] Heitman J.2010. Evolution of eukaryotic microbial pathogens via covert sexual reproduction[J]. Cell Host & Microbe, 8(1): 86-99. [13] Kim H K, Cho E J, Lee S, et al.2012. Functional analyses of individual mating-type transcripts at MAT loci in Fusarium graminearum and Fusarium asiaticum[J]. FEMS Microbiology Letters, 337(2): 89-96. [14] Kim H K, Lee T, Yun S H, 2008. A putative pheromone signaling pathway is dispensable for self-fertility in the homothallic ascomycete Gibberella zeae[J]. Fungal Genetics and biology, 45(8): 1188-1196. [15] Klix V, Nowrousian M, Ringelberg C, et al.2010. Functional characterization of MAT1-1-specific mating-type genes in the homothallic ascomycete Sordaria macrospora provides new insights into essential and nonessential sexual regulators[J]. Eukaryot Cell, 9(6): 894-905. [16] Li Y G, Jiang W Y, Zhang Q F, et al.2019. Population structure and genetic diversity of Setosphaeria turcica from corn in Heilongjiang province, China[J]. Journal of Applied Microbiology, 127(6): 1814-1823. [17] Lin X, Heitman J.2014. Mechanisms of Hmothallism in fungi and transitions between Heterothallism and Homothallism[M]. Sex in Fungi: Molecular Determination and Evolutionary Implication, Washington, D. C.: ASM Press, 35-57. [18] Mideros S X, Chung C L, Wiesner-Hanks T, et al.2018. Determinants of virulence and in vitro development colocalize on a genetic map of Setosphaeria turcica[J]. Phytopathology, 108(2): 254-263. [19] Milgroom M G, 1996. Recombination and the multilocus structure of fungal populations[J]. Annual Reviews of Phytopathology, 34: 457-477. [20] Ni M, Feretzaki M, Sun S, et al.2011. Sex in fungi[J]. Annual Review Of Genetics, 45: 405-430. [21] Nicole N, Stefanie P, 2006. A MADS box protein interacts with a mating-type protein and is required for fruiting body development in the homothallic ascomycete Sordaria macrospora[J]. Eukaryot Cell, 5(7): 1043-1056. [22] Sugimoto A, Iino Y, Maeda T, et al.1991. Schizosaccharomyces pombe ste11+ encodes a transcription factor with an HMG motif that is a critical regulator of sexual development[J]. Genes Development, 5(11): 1990-1999. [23] Turgeon B G, Bohlmann H, Ciuffetti L M., et al.1993. Cloning and analysis of the mating type genes from Cochliobolus heterostrophus[J]. Molecular and General Genetics, 238: 270-284. [24] Turina M, Prodi A, Alfen N K, 2003. Role of the Mf1-1 pheromone precursor gene of the filamentous ascomycete Cryphonectria parasitica[J]. Fungal Genetics and Biology, 40(3): 242-251. [25] Wang Z, Kin K, López-Giráldez F, et al.2012. Sex-specific gene expression during asexual development of Neurospora crassa[J]. Fungal Genetics and Biology, 49(7): 533-543. [26] Whittle C A, Nygren K, Johannesson H.2011. Consequences of reproductive mode on genome evolution in fungi[J]. Fungal Genetics and Boilogy, 48(7): 661-667. [27] Zhao Z, Liu H, Wang C, et al.2014. Comparative analysis of fungal genomes reveals different plant cell wall degrading capacity in fungi[J]. BMC Genomics, 14:274. [28] Zheng P, Xia Y L, Zhang S W, et al.2013. Genetics of Cordyceps and related fungi[J]. Applied Microbiology and Biotechnology, 97(7): 2797-2804.